嗜酸性粒细胞（Eos）浸润与慢性鼻-鼻窦炎伴鼻息肉（CRSwNP）的预后密切相关，而鼻黏膜局部Eos浸润的调控机制不明。我们前期发现：γδT细胞在CRSwNP中表达最高，且与Eos浸润程度正相关。新近发现IL-17是银屑病皮损中γδT细胞产生的重要致炎因子，据此推测：鼻黏膜局部γδ T细胞通过表达IL-17调控Eos浸润。本项目拟通过组织标本检测获得CRSwNP鼻黏膜γδT细胞与IL-17直接相关的可靠证据；然后通过致炎因子激发Tcra-/-、Tcrd-/-小鼠，采用基因扫描技术确定鼻黏膜局部产IL-17的特异性γδT细胞亚群；最后通过同源γδ T细胞优势亚群过继转移、特异性IL-17抗体封闭技术，证实特异性γδT细胞亚群通过IL-17调控Eos浸润。本项目旨在阐明CRSwNP中以γδT细胞为核心的局部免疫应答机制，为完善CRSwNP发病理论，寻求新的局部治疗方案，改善疾病预后提供科学依据

The eosinophil infiltration in nasal mucosal plays an important role in chronic rhinosinusitis with nasal polyps (CRSwNP), and is frequently associated with poor prognosis. However, the mechanism of eosinophil infiltration remains unclear. Our recent study demonstrated that there was a positive correlation between γδT cells expression and eosinophil infiltration, and the highest expression of γδT cells was found in CRSwNP, followed by that in chronic rhinosinusitis without nasal polyps and that in normal nasal mucosa. It was affirmed that γδT cells are the major producers of IL-17 in psoriasis. We suggest that γδT cells maybe regulate the eosinophil accumulation in nasal mucosa via overexpressing IL-17 in CRSwNP. The proposed study intends to evaluate the expression of γδT cells in nasal mucosa and its correlation with IL-17 by using qRT-PCR and immunohistochemistry, to comfirm that specific γδT cell subtype is the major source of IL-17 by using gene scanning in the knockout mice model, to study the potential mechanism that γδT cells regulate the eosinophil infiltration via IL-17 by using adoptive transfer and antibody blocking. The proposed study will be helpful to gain more insight into the role of γδT cells in local immune response of CRSwNP, and provide a potential approach in developing a new treatment strategy and improve the prognosis of this disease.