汗腺是皮肤最重要的附属器之一，在分泌汗液和调节体温与代谢等方面发挥重要作用。严重创烧伤后皮肤汗腺再生是组织修复和再生医学研究的重点、热点与难点。本项目基于我们前期在汗腺发育、诱导分化、关键信号通路阐明和初步临床试验取得的阶段性成果，进一步从微环境与汗腺发育和修复再生关系进行研究。重点研究汗腺发育的结构微环境和生理微环境特征，揭示不同损伤导致汗腺所处微环境变化规律，阐明损伤影响汗腺修复和再生的关键性制约因素。在此基础上利用原位诱导分化技术和3D方法，重建或恢复汗腺再生生理微环境，以实现在体和体外汗腺的完美修复与再生。整项研究一方面将进一步丰富和完善我们有关汗腺再生的原创性理论，同时将建立一套完整的重建汗腺结构与功能的方法，有望在较大程度上恢复严重创烧伤患者后期的发汗功能。研究结果也将为新一代含有附件的组织工程皮肤和其它相关产品研发提供理论支撑和技术保障。

Sweat gland is one of the most important skin appendages, which plays irreplaceable role in perspiration, body temperature adjustment and metabolism. Sweat gland and skin regeneration after severe burn injury have been the key points in tissue repair and regenerative medicine. Based on our previous achievements in sweat gland development, inducing differentiation, demonstration of key signal pathway and preliminary clinical trials, this program would like to further investigate the association between microenvironment and sweat gland development, repair and regeneration. Characteristics in structural and physical microenvironments of sweat glands development should be focused, in order to unveil the changing rules of sweat glands microenvironment after different injury, and to illustrate crucial restraining factors in sweat gland repair and regeneration. In addition, with the aid of approaches of in situ inducing differentiation and 3D bioprinting, sweat gland regenerative and physical microenvironment should be reestablished and restored, in order to achieve perfect sweat gland repair and regeneration in vivo and in vitro. The program, in one hand, will complete and enrich our innovative theories in sweat gland regeneration, and in another hand, establish integrated methods in rebuilding sweat gland structure and function, which is expected to restore the function of perspiration in patients of severe burn injury to a greater degree. The results will also provide theoretical support and technological safeguard for tissue engineering skin with appendages and other related products.