损伤皮肤修复过程中皮肤附件，特别是汗腺的再生已经成为再生医学领域研究的重点与难点。本项目基于我们前期关于汗腺发育规律、鉴别汗腺标志物的发现以及成功诱导骨髓间充质干细胞（MSCs）转变为汗腺样细胞的工作基础，通过创建新一代共培养诱导分化体系，在获得高纯度汗腺样细胞的基础上，继续深入研究MSCs重编程为汗腺样细胞过程中表观遗传学的改变及其特征、发现并鉴别出诱导汗腺样细胞重编程的关键信号通路、了解汗腺样细胞重编程相关网络调控机制、识别诱导汗腺样细胞重编程的关键转录因子、考察诱导体系的稳定性、有效性和安全性。在此基础上，进一步完善我们前期有关利用干细胞诱导分化技术再生汗腺的理论基础，并形成可为临床转化应用的关键技术。研究结果将使我国在皮肤修复与再生领域继续保持领先地位。

The regeneration of skin and its appendages such as sweat glands is one of the focuses in the regenerative medicine. Based on our previous studies in sweat gland development, biomarkers discovery for identification of sweat gland and inducing the differentiation of human bone-marrow MSCs (hMSCs) into sweat-gland-like cells (SGCs) using in vitro co-culture methods, we will explore the optimal co-culture conditions for the transdifferentiation of MSCs into SGCs. Then, we will further explore the epigenetic characteristics and key variables signal pathway in inducing the phenotype shift from MSCs into SGCs. Finally, the safety of the in vitro co-culture methods and its application will be evaluated, which will benefit a better understanding of the mechanisms underlying phenotype shift from MSCs into SGCs since some of these molecular factors are also associated with carcinogenesis. The results will offer the important theories and key skills for sweat gland regeneration and bring new hope for small organ repair and regeneration using stem cell dedifferentiation or transdifferentiation.