葛花枳椇子配伍防治大鼠慢性酒精性肝损伤的量-时-效关系研究及机制探讨

葛花与枳椇子是古代解酒方中常相须配伍使用的两味解酒专药，研究表明二者具有较好的解酒、抗氧化作用。前期工作显示，酒精性肝损伤大鼠肝组织出现脂肪变性及炎症浸润，葛花枳椇子2:1配伍解酒保肝的作用优于单味药的疗效，能够降低血脂含量，对肝组织炎症及脂肪变性的病理变化均有改善作用。因此，本课题拟以酒精性脂肪肝和肝炎为研究对象，通过在体、离体实验相结合，从配伍剂量，给药周期两个角度，探讨葛花枳椇子配伍的量-时-效关系，阐明葛花枳椇子最佳配伍条件，并进一步通过 "酒精－内毒素－kupffer细胞－TNFα-肝脏炎症-细胞凋亡途径"以及脂质代谢途径，揭示葛花、枳椇子配伍后对酒精性脂肪肝和酒精性肝炎的影响及作用机制，以期为临床医师应用葛花枳椇子防治酒精性肝损伤提供借鉴，为阐明葛花枳椇子解酒保肝的作用机制提供依据。

The Flos Puerariae often used by compatibling with seeds of Hovenia dulcis Thunb in the ancient time.Previous studies have shown that the Flos Puerariae and seeds of Hovenia dulcis Thunb have good function of hangover and antioxidant effect.Preliminary work shows that the liver in the rats with alcoholic liver injury will have fatty degeneration and infiltration of inflammatory cells.Detection indicators from the previous experiments, such as liver function indicators, the ethanol concentration in the blood, liver alcohol dehydrogenase activity and liver pathological form ,we can know that the group of 2:1 has the best effect.Therefore, this project intends to study alcoholic fatty liver and hepatitis, though vivo and vitro experiments,from the two angles of compatibility dose and dose cycle,we will explore the dose-time-effects and mechanism of compatibility Flos puerariae and seeds of Hovenia dulcis Thunb in alcoholic liver disease rat.In order to explain best compatibility condition of Flos puerariae and seeds of Hovenia dulcis Thunb，and further through the "alcohol-endotoxin-kupffer cell-TNFα-liver inflammation- apoptosis"pathway and lipid metabolism pathway to reveal the Effect and action mechanism of alcoholic liver disease(ALD by Flos puerariae and seeds of Hovenia dulcis Thunb.We hope that this project could provid reference for clinicians who will use Flos puerariae and seeds of Hovenia dulcis Thunb to treat alcoholic liver disease and elucidate the mechanism of hangover hangover of Flos puerariae and seeds of Hovenia dulcis Thunb.