班氏跳小蜂Aenasius bambawalei是重要入侵害虫扶桑绵粉蚧Phenacoccus solenopsis的优势伴迁天敌，野外寄生率达60%以上，应用前景广阔。室内饲养条件下，该蜂存在子代偏雄化现象。本课题组前期研究发现，36℃高温条件下该蜂的子代雌性比例可提高45.5%，且其体内葡糖脱氢酶Glucose dehydrogenase(GLD)表达量显著上调。GLD可参与精子的吸收和释放，与寄生蜂的性别分配密切相关。因此，研究高温介导GLD的作用机制，对于解决该蜂偏雄化问题具有重要指导意义。本项目将1）应用CRISPR9基因编辑方法，分析GLD功能；2）明确GLD参与的信号通路在性别分配中的作用；3）论证高温介导与GLD表达的关系。以上研究结果既有助于揭示班氏跳小蜂性别分配的内在机制，又可为解决该蜂偏雄化问题提供重要科学依据，以利于其规模化饲养及利用。

The invasive mealybug Phenacoccus solenopsis (Hemiptera: Pseudococcidae) has been recognized as an important pest in China, and Aenasius bambawalei (Hymenoptera: Encyrtidae) is the predominant accompanying parasitic wasp of this mealybug. Moreover, as an excellent biocontrol agent, the parasitization rate of this parasitic wasp could up to more than 60% in field. However, in the mass rearing, there are more male offspring in the population. Our previous findings have shown that 36 ℃could significantly enhance the proportion (45.5%) of female offspring of A. bambawalei . In addition, the expression of Glucose dehydrogenase (GLD) gene in this temperature is the most. On the other hand, GLD could facilitates both sperm uptake and release , and plays a critical role in the sex allocation. Thus, make clear the mechanism of high temperature mediate GLD for sex allocation of A. bambawalei is very important for solving the problem of more male offspring. In this study, 1)CRISPR/Cas 9 gene edit technology will be used to tested the comprehensive function of GLD; 2) the function of signal pathway which GLD involved in will be verified ; 3) mechanism of sex allocation resulted from the role of high temperature on GLD expression will be demonstrated. Our expected findings will uncover the mechanism involved in sex allocation in A. bambawalei, and on the other hand, will helpful for solving the problem of more male offspring in future.