脊髓损伤（SCI）后多因素动态病理变化已成为脊髓再生修复治疗和功能重建的瓶颈，目前尚无有效治疗手段。因此有效对接SCI动态病理变化的多靶向治疗对脊髓再生修复尤为重要。我们前期研究发现嗅鞘细胞（OEC）在CCM等分子持续作用下其吞噬免疫调节、增殖及分泌功能显著放大，精原干细胞（SSC）在特定条件下可被诱导分化为脊髓神经元。本项目拟以OEC和SSC为切入点，采用SAP包裹负载CCM等分子PLC生物材料缓释诱导OEC并联合SSC定向分化的综合方案，研究其对SCI动态病理变化的多靶向治疗作用。进一步通过细胞分子生物学及功能学分析等相关技术深入探讨此治疗体系对SCI动态病理变化的多靶向治疗促脊髓再生和功能重建分子机制。最终建立高效促脊髓再生多靶向精准治疗的新技术平台，为临床上诊治SCI动态病理变化提供了新的思路和手段。同时本项目将阐明多靶向治疗SCI的调控机制，为有效治疗SCI提供新的分子机制。

An extremely multifaceted dynamic pathological change following spinal cord injury (SCI) has been regarded as a significant bottleneck in spinal cord regeneration and function repair. Until now, there are no effective treatments. Therefore, it is pivotal solution to seek an efficient strategy to butt multitherapeutic targeting and the multifaceted pathological process to overcome the overwhelming issue, leading to remarkable improvement of SCI and function recovery. Fascinatingly, our recent studies demonstrated that olfactory ensheathing cells following a consecutively medicinal treatment such as CCM, can be activated and further maximize its cellular biofunctions including phagocytosis, immunimoduation, proliferation and secretion. Combinationally, spermatogonial stem cells (SSCs) can be converted into spinal cord neural cells under involvement of a set of defined growth factors. On the basis of the results obtained from previous project, the aims of our present project are to systematically investigate the combinatorial therapeutic roles in the multifaceted dynamic pathological change following SCI by means of neurodifferentiated SSCs and activated OECs caused by a set of bioactive molecules released from polylactic-co-caprolactone (PLC) fibrous scaffold coated by SAP. Moreover, the assessment of neural regeneration and axonal outgrowth following treatment of the combinational strategies was conducted by means of morphological, cellular and molecular, physiological animal behavior assays, and the possible mechanisms underlying the targeting strategies to treat SCI with progressive multifaceted pathology was concomitantly investigated and clarified. We will eventually develop an efficient technology platform for both multi-targeting accurate treatment of SCI and improvement of functional outcomes following lesion. Thus the present project will provide an alternative therapeutic solution for neural regeneration by the clinical application of comprehensive treatments of multifaceted SCI dynamic pathology, and elucidate the underlying mechanisms by which multitherapeutic targeting to treat SCI with progressive multifaceted pathological change results in spinal cord regeneration Meanwhile, On the other hands, it may provide novel theoretical basis for clarification of the precise molecular mechanisms in future clinical application.