从LncMEG3调控卵巢颗粒细胞自噬探讨针刺对PCOS排卵障碍效应机制研究

卵泡发育异常是多囊卵巢综合征排卵障碍的主要病理因素，而卵巢颗粒细胞自噬过度激活是卵泡发育异常的重要因素。研究表明，LncMEG3通过PI3K/AKT/mTOR调控自噬，但此调控机制在卵巢颗粒细胞中是否成立尚未明确。预实验发现LncMEG3在PCOS大鼠卵巢颗粒细胞中低表达，针刺干预能上调其表达。据此我们假设，针刺可能影响LncMEG3表达，介导PI3K/AKT/mTOR调控卵巢颗粒细胞自噬，促进卵泡发育而达到治疗PCOS排卵障碍作用。本研究建立PCOS大鼠模型，开展以下研究①观察针刺干预PCOS大鼠后卵泡发育情况，明确针刺治疗PCOS排卵障碍的疗效；②检测MEG3，PI3K，AKT，mTOR，LC3II/I与p62，明确PCOS发病机制；④检测针刺后上述指标，并与②对比，探讨针刺治疗PCOS排卵障碍机制。本研究将从LncMEG3这个新视点为揭示针刺治疗PCOS排卵障碍的效应机制提供新思路。

Follicular dysplasia is the main pathological factor of ovulation dysfunction in polycystic ovary syndrome, and over-activation of autophagy in ovarian granulosa cells is an important factor in follicular dysplasia.Studies have shown that LncMEG3 regulates autophagy via PI3K/AKT/mTOR. However, whether this regulation mechanism in ovarian granulosa cells is established is not known. Consequently, we designed pre-experiments that LncMEG3 was down-regulated in PCOS ovarian granulosa cells, and acupuncture intervention could up-regulate the expression of LncMEG3 in ovarian granulosa cells of PCOS rats. Based on this hypothesis, acupuncture may affect the expression of LncMEG3, mediate PI3K/AKT/mTOR to regulate autophagy in ovarian granulosa cells, promote follicular development, and thus achieve the role of treatment of PCOS ovulation dysfunction. This study planes to establish a rat model of PCOS and carry out the following studies : ①to observe the follicular development after acupuncture intervention in PCOS rats to definite curative effect of acupuncture in the treatment of PCOS; ②to detect LncMEG3, PI3K, AKT, mTOR, LC3II/I and p62, so as to clarify the pathogenesis of PCOS; ③to detect the above indicators after acupuncture intervention, and to compare with these indicators of the 2nd items, for exploring the mechanism of acupuncture treatment of PCOS.This study will provide a new idea from the new viewpoint of LncMEG3 to reveal the mechanism of acupuncture treatment of PCOS.