C型产气荚膜梭菌引起的仔猪腹泻对养猪业危害严重，研究报道ssc-let-7i-TARBP2与宿主抵抗病原菌感染的肠道炎症相关。我们前期发现C型产气荚膜梭菌易感和耐受仔猪间差异表达lnc001186与ssc-let-7i存在结合位点，我们推测lnc001186可能通过吸附ssc-let-7i调控TARBP2参与C型产气荚膜梭菌感染。因此，本项目通过过表达、抑制和双荧光素酶报告系统，研究ssc-let-7i与TARBP2的调控关系及其对C型产气荚膜梭菌感染的调控功能；结合双荧光素酶报告系统和RIP验证lnc001186/ssc-let-7i/TARBP2的结合位点及调控关系；通过过表达ssc-let-7i和lnc001186，验证ceRNA网络的调控作用。通过以上研究探索lnc001186/ssc-let-7i/TARBP2在C型产气荚膜梭菌性仔猪腹泻的作用机制，为腹泻抗性猪品种选育提供参考。

Clostridium perfringens (C. perfringens) type C-infected piglet diarrhea lead to serious loss to pig industries. Study has reported ssc-let-7i-TARBP2 was close related with intestinal inflammatory diseases of hosts resistant against pathogen infection. In our previous study, different expressed lnc001186 and ssc-let-7i of resistant and susceptible piglets during C. perfringens type C infection had the potential target binding sites, we speculate that lnc001186 may regulate target gene TARBP2 by adsorbing ssc-let-7i, which plays an important regulatory role in the diarrhea of piglets infected with C. perfringens type C. In this project, overexpressing and silencing and dual luciferase reporter assay were used to analyze the relationship and regulatory functions of ssc-let-7i and TARBP2 on C. perfringens type C-infected IPEC-J2 cell; the binding sites and regulation relationships of lnc001186/ssc-let-7i/TARBP2 will be certified using dual luciferase reporter assay and RNA binding protein immunoprecipitation; the regulation function of ceRNA network will be certified by co-overexpressing ssc-let-7i and lnc001186 in IPEC-J2 cell. The findings will reveal the regulation mechanism of lnc001186/ssc-let-7i/TARBP2 network in piglet diarrhea caused by C. perfringens type C and provide references for the selective breeding of diarrhea-resistant pig breeds.