人体衰老是事关国计民生的重大课题，皮肤衰老是整形外科学学科领域中的重要科学问题，但缺乏有效的干预措施。申请人前期研究表明，长链非编码RNA(Long-noncoding RNA, LncRNA) MALTA1与皮肤衰老相关，其调节衰老的机制主要通过TIMP蛋白及其他细胞膜结合蛋白。然而，TIMP的调节作用存在抑制或者促进衰老的多向性。申请人发现，结合锚定蛋白GPI的TIMP1能够抑制细胞增殖，促进凋亡。因此，申请人提出，MALTA1通过TIMP1调节皮肤衰老的过程，能够被结合了锚定蛋白GPI的TIMP1所改变，从而达到抗衰老的效果。本项目拟通过细胞学实验、动物模型实验，运用转基因技术、基因测序等手段，揭示MALTA1通过TIMP1-GPI干预皮肤衰老的分子机制，初步阐明TIMP1和其他膜结合蛋白及其下游分子参与皮肤衰老的作用机制，为临床抗衰老医学实践提供借鉴。

Aging is one of the major problems of the society, and skin aging is the key scientific issue in plastic surgery research area, but it lacks strategies. According to our previous studies, Long-noncoding RNA-MALTA1 was related to skin aging, and its aging-regulation mechanism was mediated by TIMP and other cell-membrane connected proteins. However, the regulation effects of TIMPs showed multi-directional as inhibit or promote aging. As has been demonstrated by us, when TIMP1 combined with GPI, which is a cell-membrane anchor protein, they can inhibit cell proliferation, and promote apoptosis. Therefore, we hypothesize that MALTA1 regulates aging through TIMP1, which can be changed by TIMP1-GPI, and then influence the effects of anti-aging. So we plan to design the cell experiment, animal experiment, apply the trans-gene technology, gene-sequencing technology, try to figure out the mechanism of MALTA1 regulating skin aging through TIMP1-GPI, TIMP1 and other cell-membrane connected protein attending skin aging, and provide the new method for clinical anti-aging practice.