大麦半矮杆基因ari-e.GP的克隆及其对产量和耐盐性状的影响研究

半矮杆品种抗倒性强、耐重施肥和高种植密度，有利于高产和机械化收获。ari-e.GP是大麦半矮杆育种的重要基因。携带该基因的品种Golden Promise广泛用于大麦半矮杆育种。除影响株高外，它还与产量、耐盐性相关。该基因已用不同的标记进行定位和QTL分析，但尚未克隆。利用Dash/VB9104DH群体研究发现在ari-e.GP基因区域检测到株高主效QTL，同时检测到产量QTL。随着大麦基因组的公布，可望通过图位克隆法获得ari-e.GP。本研究采用Dash/VB9104 DH群体和Dash×WB2484回交群体精细定位并克隆ari-e.GP，开发基因标记为育种所用。利用Golden Promise进行功能互补试验，阐明ari-e.GP基因的半矮机制。通过对转基因与非转基因大麦的产量和耐盐性试验，进一步揭示ari-e.GP基因对产量和耐盐性的影响。本研究可为大麦半矮杆育种提供理论依据。

Semi-dwarf plants improved harvest index resulted from more resistant to lodging, suitable to heavy fertilizer application and dense field planting. As a result, they increased grain yield and adapted to mechanize harvesting. Some of the semi-dwarf genes have been depolyed in modern barley breeding. ari-e.GP is one of the most important semi-dwarf genes. The famous barley Golden Promise carried ari-e.GP gene are in the pedigree of majority of modern barley cultivars. Besides its effect on reduced height, ari-e.GP also associated with high yield and salt tolerance. The ari-e.GP gene has not yet been cloned althoght it was recently mapped as a height QTL using different kinds of markers. A major QTL for plant height accounts for 40% of the phenotypic variation was mapped to the ari-e.GP gene region in the Dash/VB9104 barley double haploid populattion. Furthermore, a QTL for yield was also identified at the same region. With the available of barley genome sequence, ari-e.GP gene can be isolated from map-based cloning. In this project, ari-e.GP gene will be fine mapped and identifed using Dash/VB9104 double haploid population and Dash/WB2484 backcross population. Gene markers will be developed for marker assited selection of barley. Golden promise was transformated with a construct containing-full length ari-e.GP genomic DNA,confirming that this locus is responsible for the semi-dwarf phenotype. Meanwhile,the transformation and untransformation barley will be determined their yield and response to salt stress, which will demonstrate its association between the semi-dwarf gene and yield and salt tolerance. The outcome of the project will hopefully provide the theoretical basis for barley semi-dwarf breeding.