作为一类新型生物型污染物，抗生素抗性菌（ARB）对人类健康威胁已被广泛认同，但生物暴露ARB后的毒性反应和详细体内抗性传递过程与影响仍未知。课题组前期从养殖区地下水中分到一株抗多种抗生素的志贺氏菌，分别接触暴露罗非鱼和热带爪蛙，呈现鱼肠菌群多样性明显减少和爪蛙敏感症状。在此基础上，我们提出“外源ARB暴露不仅对热带爪蛙胚胎发育存在毒性效应，而且能通过ARGs放大和水平迁移的分子机理影响热带爪蛙肠道菌群结构和进化”的研究假想。本项目将从个体、菌群、细胞和基因水平，结合体内和体外实验，利用显微观察、活体成像、组织切片、基因检测、测序、荧光示踪及现代仪器分析等手段，发现外源ARB对热带爪蛙胚胎发育的毒性效应关系，阐明外源（多重）抗性向肠道菌群传递的分子机理，希望提出抗性迁移的新方式，总结出传递的路线和规律，为外源ARB的人体健康风险防范和疾病治疗提供理论依据和技术基础。

As a kind of novel biological pollutant, the antibiotics resistant bacterium (ARB) had been considered as a human health-threatening, but the detailed in vivo pathway and toxic effects after body exposure were kept unknown. In our previous research, a Shigella flexneri strain from groundwater of livestock farm could resist to multiple antibiotics, the intestinal flora of Tilapia mossambica exposed to this strain was affected obviously and Xenopus tropicalis was sick after exposure to the strain. Based on it, we further hypothesize that the exposure of foreign ARB might be toxic to the embryonic development as well as affecting the intestine flora evolution and construction of Xenopus tropicalis by ARGs amplification and transfermation. We aim to reveal the toxic effect relationship between foreign ARB and embryonic development and reveal the molecular mechanism of foreign ARGs delivering to intestinal flora. We hope to make a breakthrough on resistance transfermation style, pathway and regularity and ultimately provide theoretical foundation and technological means for the research on human being.