dax1介导RA信号调控尼罗罗非鱼减数分裂起始的分子机制研究
结题报告
批准号:
32002367
项目类别:
青年科学基金项目
资助金额:
24.0 万元
负责人:
石红娟
依托单位:
学科分类:
水产生物繁殖与发育
结题年份:
2023
批准年份:
2020
项目状态:
已结题
项目参与者:
石红娟
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中文摘要
生殖细胞减数分裂是有性生殖生物配子形成的关键,调控减数分裂的分子机制是生物学研究热点之一。尼罗罗非鱼是世界性养殖鱼类,前期研究证明视磺酸RA是调控罗非鱼减数分裂起始的关键因子,但其分子机制尚不清楚。申请人发现RA能剂量依赖地激活罗非鱼性腺中dax1的表达,且敲除dax1导致XX和XY罗非鱼减数分裂起始受阻。这说明dax1可能作为RA的靶基因调控减数分裂起始,但其机制亟待阐明。为此,本项目拟开展以下研究阐明RA-dax1途径调控罗非鱼减数分裂起始的分子机制:1)通过在体、离体实验证实RA对dax1表达的转录调控;2)基于dax1纯合敲除鱼的性腺表型,阐明RA靶基因dax1在调控减数分裂起始中的作用;3)通过性腺ChIP-Seq及性腺转录组测序筛选、鉴定dax1的下游靶基因,加以过表达回救dax1敲除鱼验证功能。本项目有助于丰富人们对鱼类减数分裂机制的认识,进而为鱼类人工繁殖提供理论基础。
英文摘要
In sexually reproducing organisms, meiosis is a germ cell-specific division that is indispensable for the generation of haploid gametes. The study of regulatory mechanisms in meiosis is always an important research area in biology. Previous study in Nile tilapia indicated that retinoic acid (RA) plays a critical role in regulating the entry of germ cells into meiosis. To date, the regulatory mechanism of RA pathway in meiosis initiation remains unclear. Our previous studies demonstrated that RA dosage-dependently stimulates the expression of gonadal dax1 (dosage sensitive sex reversal (DSS), adrenal hypoplasia congenital (AHC) critical region on the X chromosome, gene 1) in tilapia. More importantly, the meiosis initiation was delayed in dax1 homozygous (dax1-/-) XX and XY fish, compared to that of the wild-type (dax1+/+) fish. The results indicated that dax1 might function as a target gene of RA to regulate meiosis initiation. Based on these results, our project is targeted to clarify the regulatory mechanisms of RA-dax1 pathway in fish meiosis initiation following three aspects as follows: 1) to confirm the transcription regulation of RA on dax1 by promoter analysis using a dual-luciferase reporter system and electrophoretic mobility shift assay; 2) to confirm the roles of RA target gene dax1 in regulating meiosis initiation, based on the gonadal phenotype of dax1 homozygous mutants; 3) to perform gonadal transcriptomic sequence and ChIP-Seq to screen and identify the target genes regulated by Dax1, followed by overexpression of target genes in dax1 mutants. Our research will extend the comprehension of the regulatory mechanism of meiosis initiation in germ cells. Moreover, our study will provide a theoretical foundation for artificial propagation in aquaculture.
期刊论文列表
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科研奖励列表
会议论文列表
专利列表
DOI:doi: 10.3390/ijms242015356.
发表时间:2023
期刊:International Journal of Molecular Sciences
影响因子:--
作者:Yongguan Liao;Hongjuan Shi;Tong Han;Dongneng Jiang;Baoyue Lu;Gang Shi;Chunhua Zhu;Guangli Li
通讯作者:Guangli Li
DOI:10.1016/j.aquaculture.2023.739381
发表时间:2023-02
期刊:Aquaculture
影响因子:4.5
作者:Sen Fan;Hong‐Juan Shi;You-Xing Peng;Yuanqing Huang;Mouyan Jiang;Guang-Li Li-Guang-Li-Li-2151297544;De-shou Wang;
通讯作者:Sen Fan;Hong‐Juan Shi;You-Xing Peng;Yuanqing Huang;Mouyan Jiang;Guang-Li Li-Guang-Li-Li-2151297544;De-shou Wang;
DOI:10.1111/jwas.12815
发表时间:2021-06
期刊:Journal of the World Aquaculture Society
影响因子:2.8
作者:Dongneng Jiang;Ziming Kuang;Kong-song Yang;Yuanqing Huang;U. Mustapha;Xiangbo Guo;De‐Feng Zheng
通讯作者:Dongneng Jiang;Ziming Kuang;Kong-song Yang;Yuanqing Huang;U. Mustapha;Xiangbo Guo;De‐Feng Zheng
DOI:https://doi.org/10.1016/j.cbd.2021.100949
发表时间:2021
期刊:Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics
影响因子:--
作者:Hongjuan Shi;Xiaoying Ru;Shuhui Pan;Dongneng Jiang;Yang Huang;Chunhua Zhu;Guangli Li
通讯作者:Guangli Li
DOI:10.7541/2023.2022.0286
发表时间:2023
期刊:水生生物学报
影响因子:--
作者:潘书慧;周彤;袁嘉慧;罗琼;秦若彤;茹笑影;黄洋;石红娟;李广丽
通讯作者:李广丽
国内基金
海外基金