我国在生物制氢基础理论和工程技术领域已经处于国际领先地位，基于代谢工程理论的产氢细菌改造成为进一步提升生物制氢技术水平和降低制氢成本的关键。秸秆能够为生物制氢提供廉价、丰富、可靠的原料，而其主要成分木糖的高效利用是提高秸秆利用效率、降低制氢成本的前沿课题，符合国家“十三五”科技创新规划和生态环境保护的战略需求。本项目拟通过代谢工程技术手段，优化遗传操作工具，筛选合适的木糖代谢基因，在哈尔滨产乙醇杆菌中构建木糖代谢途径，获得高效木糖产氢工程菌株；调控影响工程菌生长和代谢条件，优化木糖工程菌产氢效率，解析工程菌代谢物阻遏效应，利用基因组、转录组等手段解析生物学机制，建立混合糖高效代谢模型；构建木糖工程菌利用秸秆水解液的发酵产氢工艺，有效解决秸秆利用的瓶颈问题，实现农业废弃物高效资源化能源化，最大限度地降低制氢成本，为氢能大规模生产提供技术支撑，为我国能源战略转型和生态环境保护做出积极贡献。

China has taken the leading position worldwide in fundamental theory and engineering technology for biological hydrogen production. The reconstruction of hydrogen-producing bacteria based on the metabolic engineering theory has been the key to further stimulate technological development and cut costs of biological hydrogen production. Straws are cheap, high-available and reliable feedstocks for biohydrogen production. However, xylose is the main composition of straws. Thus, the efficient utilization of xylose is a cutting-edge topic to improve energy recovery from straws and further save the cost of hydrogen production, which meets the strategic demands of scientific and technological innovation ecological environment protection of the 13th national five-year in China. This project will optimize the genetic manipulation tools for Ethanoligenens harbinense, screen the suitable xylose metabolism genes, and then construct the xylose metabolic pathway in Ethanoligenens harbinense. This project will obtain the engineering strain with high performance for hydrogen production from xylose. The operation parameters influencing the cell growth and xylose metabolism will investigated and optimised, and metabolites repressor effect on engineering bacteria will be revealed. The genomics and transcriptomics will be applied to investigate the molecular mechanism of the mixed sugar utilization mechanisms and establish the metabolism model of the engineered strain. Finally, hydrogen production process from straw hydrolysate will be developed with the engineered E. harbinense, which will break through the bottleneck of straw utilization. This project will enable efficient resource and energy recovery from agricultural waste, minimize hydrogen production costs and provide technical support for large-scale hydrogen production, which will make positive contributions to China's energy strategic transition and ecological environment protection.