氨基甙类抗生素（AmAn）耳毒性损害病机错综复杂，是因聋致残的主要原因之一。前期研究提示，其与氧化应激（OS）损害密切相关，最新研究表明，国家专利健耳方具有显著防护动物庆大霉素（GM）耳毒性作用，其机制与抑制OS反应损害有关，但调控机制尚未明了，据此提出新的假说，P38/JNK-MAPK、FasL-Fas、Cytc-Apaf不同径路与OS反应相关，干预和影响这些径路的某些特异性因子的正反联动关系是AmAn耳毒性损害机制重要环节，中药通过靶向影响这些因子的联动关系，起到防治AmAn耳毒性的作用。鉴此，我们将继续利用以GM为代表的AmAn耳毒性损害动物模型，选择新一代转录组学，配合RT-PCR、免疫荧光分子探针、耳蜗铺片、扫描电镜等技术和方法，探索和明确不同径路调控OS反应靶点及相互关系，进一步揭示AmAn耳毒性发病机制和中药作用机制，为发展中医治聋理论，开发防聋治聋中药提供新的科学依据。

The pathogenesis of aminoglycoside antibiotics(AmAn)-induced ototoxic damages is very complex. It is one of main causes of deafness-induced disability. Previous research suggest that the AmAn ototoxicity was closely related to oxidative stress (OS) damage. The national patent prescription of traditional Chinese medicine(TCM) had significant protective effects against animal gentamicin (GM)-induced ototoxicity. Its mechanism of action was related to inhibiting OS response and preventing programmed cell death mediated by Caspase, however, there are many factors to affect OS response, and the regulatory mechanism is not yet clear. Accordingly, propose a new hypothesis that different pathways of P38/JNK-MAPK, FasL-Fas and Cyt c-Apaf are related to OS response. The positive and negative linkage of some specific factors that interfere in and influence these pathways are an important link in the mechanism of AmAn –induced ototoxic damage. The TCM will play a role in preventing and curing AmAn ototoxicity by affecting the positive and negative linkage of these targeted factors. In view of this, we will continue to use the animal models of AmAn ototoxic damage which is represented by GM, select the techniques and methods such as new level transcriptomics, RT-PCR, western blot, immunofluorescence molecular probe, cochlear basilar membrane as flat surface preparations, scanning electron microscopy and so on, investigate and clear OS response targets and their relationships in different pathways regulation, further elucidate the pathogenesis of AmAn ototoxicity and the protective mechanism of TCM against AmAn ototoxic deafness, and provide a new scientific basis for developing the theories of treating deafness with TCM and new drugs of TCM in preventing and treating deafness.