本项目基于前期苯丙素类杀灭单殖吸虫构效关系研究基础上，以6种杀虫高活性苯丙素类衍生物、小林三代虫模型为材料进行作用靶标研究。首先以三乙二醇为起始，通过与苯丙素类衍生物进行缩合、取代、酯化等一系列化学反应得到硫辛酸类苯丙素化合物。然后将三水合氯金酸和硼氢化钠反应，进一步采用静电吸附将两者共价结合制备含苯丙素类衍生物的金纳米探针，并与小林三代虫裂解液孵育，经SDS-PAGE分离、MALDI-TOF/TOY 质谱进行靶蛋白鉴定、RNA干扰验证；在此基础上，通过光谱学方法研究苯丙素类化合物和靶蛋白的相互作用模式，为构建计算机辅助药物设计模型提供信息。本研究不仅为揭示杀虫机制提供理论依据，而且为有目标的结构改造和优化苯丙素类衍生物，获得理化性质、药理活性等均有大幅提升的临床候选药物，创制具有我国自主知识产权的渔用原料药，有着重要的科学理论意义和应用价值。

Based on the previous studies of the relation between phenylpropanoids and monogenea, this project is focused on finding the targets of the six highly active derivatives against Gyrodactylus kobayashii. Firstly, triethylene glycol and phenylpropanoids were used to synthesize thioctic connective intermediate compound through a series of chemical reactions such as condensation, substitution and esterification. Then gold nanoparticles were synthesized by reaction of gold acid chloride trihydrate and sodium borohydride. Furthermore, it will be electrostatic adsorption generated phenylpropanoids connected gold nanoprobes. Using the nanoprobes, the binded proteins of test compounds were pulled down from the cellular of G. kobayashii proteome through SDS-PAGE separation. The target proteins can be identified by MALDI-TOF/TOY mass spectrometry, then targets of phenylpropanoids will be validated by RNA interference and bioinformatics analysis. Based on above results, spectroscopic methods will be used to study interaction modes between phenylpropanoids and target proteins. All the research work will not only provide theoretical foundation for insecticidal mechanism study, but also be meaningful for acquiring clinical drug candidates with physicochemical property and pharmacological activity highly raised through next constructing computer aided drug design mode and purposeful modify and optimize the structure of phenylpropanoids, which lays the academic foundation important application prospect for creating fishery active pharmaceutical ingredient that possess patent of China.