Effect of a new arctigenin derivative against Gyrodactylus kobayashii through inhibition of ATP production
Effect of a new arctigenin derivative against Gyrodactylus kobayashii through inhibition of ATP production
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新型牛蒡甙元衍生物通过抑制 ATP 产生对小林环指藻的作用
DOI：
10.1016/j.aquaculture.2021.736671
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发表时间：
2021-03
期刊：
影响因子：
4.5
通讯作者：
Liu Tianqiang
中科院分区：
文献类型：
--
作者：
Tan Xiaoping;Hu Yang;Qu Shenye;Wang Gaoxue;Liu Tianqiang
有毒病原体往往导致高死亡率，给水产养殖带来严重的经济损失。目前，圆指病的治疗主要依靠未经批准的化学品，但效果并不总是令人满意。根据先前的研究，牛蒡甙元是一种有效控制圆指病的天然产物。为了寻求高效化合物，合成了一种新的牛蒡甙元衍生物（CCA）。接下来，我们评估了CCA的体外和体内驱虫活性，并研究了其作用机制。与对照组相比，Gyrodactyluskobayashi的数量显着减少，CCA处理后24和48 h的EC50值分别为0.616和0.451 mg/L，EC100在CCA浓度为3 mg/L的浴处理1 h后。体外结果表明，CCA可以快速减少G的数量。小林并抑制其移动性，这通常会导致死亡。 CCA的浓度和持续时间与G的死亡率呈正相关。小林。此外，CCA 对 G 表现出 100% 的功效。仅暴露 20 分钟后，kobayashiiat 为 4.0 mg/L。急性毒性试验表明，CCA对金鱼的LC50为5.012 mg/L，是EC50的8.13倍。此外，共聚焦显微镜研究的结果表明，CCA导致蠕虫的表皮结构和肌肉溶解。此外，在体外和体内接触CCA后，蠕虫的ATP水平均急剧下降。 CCA对蜗牛的驱虫机制与表皮结构损伤和能量不足有关。从之前的研究来看，CCA对G的EC50。小林菌（0.616 mg/L）远低于牛蒡甙元的 EC50（1.85 mg/L）（Tu et al., 2018）。综上所述，CCA比牛蒡甙元更有效，在控制轮指感染方面具有巨大潜力。
Noxious pathogens always lead to high rates of mortality, which results in severe economic losses in aquaculture. Currently, gyrodactylosis treatments mainly rely on unapproved chemicals, which are not always satisfactory. According to the previous study, arctigenin is an efficient natural product for controlling gyrodactylosis. For the sake of seeking high-efficiency compounds, a new arctigenin derivative (CCA) was synthesized. Next, we evaluated the anthelmintic activity of CCAin vitroandin vivo, and studied the mechanism of action. The number ofGyrodactyluskobayashiisignificantly reduced as compared with the control group, the EC50value at 24 and 48-h after CCA treatment was 0.616 and 0.451 mg/L, respectively, with EC100at CCA concentration of 3 mg/L after 1 h bath treatment.In vitroresults showed that CCA can rapidly reduce the amount ofG. kobayashiiand inhibit its mobility that could normally lead to death. The concentration and duration of CCA were positively correlated with the mortality ofG. kobayashii. In addition, CCA displayed 100% efficacy againstG. kobayashiiat 4.0 mg/L after only 20 min exposure. Acute toxicity assay demonstrated that the LC50of CCA against goldfish was 5.012 mg/L, which was 8.13 times higher than that of the EC50. Furthermore, the results of confocal microscope study revealed that CCA leaded to the epidermal structure and dissolved muscles of worms. Moreover, the ATP level of worms were decreased sharply after bothin vitroandin vivoexposures with CCA. The anthelminthic mechanism of CCA against gyrodactylids was related to epidermal structure damages and energy insufficient. From the previous study, the EC50of CCA againstG. kobayashii(0.616 mg/L) was much lower than the EC50(1.85 mg/L) of arctigenin (Tu et al., 2018). In summary, CCA is more effective than arctigenin and has great potential in control ofGyrodactylusinfections.
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DOI：
10.3390/molecules21020228
发表时间：
2016-02-18
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Tsay SC;Lin SY;Huang WC;Hsu MH;Hwang KC;Lin CC;Horng JC;Chen IC;Hwu JR;Shieh FK;Leyssen P;Neyts J
通讯作者：
Neyts J
檀香（檀香科）对金鱼单殖感染的驱虫功效
影响因子：
2
作者：
Ling Fei;Huang Aiguo;Zhang Qizhong;Wang Gaoxue
通讯作者：
Wang Gaoxue
影响因子：
3.4
作者：
Chimerel, Catalin;Field, Christopher M.;Pinero-Fernandez, Silvia;Keyser, Ulrich F.;Summers, David K.
通讯作者：
Summers, David K.
影响因子：
3.7
作者：
Denholm SJ;Norman RA;Hoyle AS;Shinn AP;Taylor NG
通讯作者：
Taylor NG
影响因子：
2.6
作者：
Ling, Fei;Wang, Jian-Guo;Gong, Xiao-Ning
通讯作者：
Gong, Xiao-Ning