热带森林是土壤氧化亚氮（N2O）的重要排放源。西双版纳热带森林是我国蚂蚁分布最丰富的区域，蚂蚁筑巢定居通过影响巢穴地土壤微生物生物量、微生物多样性及土壤理化环境，调控N矿化、硝化/反硝化等土壤生物与生态学过程，从而影响土壤N2O的排放。以不同演替阶段4种热带森林为研究样地，研究蚂蚁多样性与蚁丘分布对土壤N2O排放的影响；原位测定蚂蚁巢穴与非巢地土壤N2O排放，并分析蚂蚁引起土壤理化性质及微生物改变对N2O排放的影响；研究蚂蚁对土壤N矿化的影响以及N矿化对硝化/反硝化底物释放的调控；研究蚂蚁引起土壤硝化/反硝化的改变对N2O排放的影响。通过以上研究，试图揭示蚂蚁筑巢定居影响热带森林土壤N2O排放的生态学过程及机制。

The tropical forest is an important source of soil N2O emission. The ant in the tropical rain forest of Xishuangbanna is the most abundant in China, and the nesting activity and settlement of ant in the forest affects soil microbial biomass and diversity, and soil physicochemical environment, which can regulate soil microbial ecology processes such as N mineralization, nitrification and denitrification. Therefore, this can have a crucial influence on the soil N2O emission. Four tropical forests at different succession stages are to be sampled for experiment to examine the effect of ant diversity and ant mound distribution on soil N2O emission; We will compare the soil N2O emission from the soils of ant nest and near the nest, and to analyze the effects of soil physicochemical properties and soil microbial change caused by the ant nesting activity on the soil N2O emission; Soils from ant nest and near nest are to be collected to explore the impact of ant nesting on soil N mineralization and that of N mineralization on the release of nitrification/denitrification substrate; We will also distinguish the change contribution of soil nitrification and denitrification on soil N2O emission under the nesting activity. Therefore, the results can determine the effect mechanism of ant nesting activity and settlement on soil N2O emissions in the Xishuangbanna tropical forests.