乙烯影响植物生长发育的多个环节以及植物对生物与非生物胁迫的响应。乙烯受体是感受乙烯、开启乙烯信号转导的首要因子，明晰其调节机制是了解乙烯信号转导的重要内容。之前仅发现乙烯受体ETR1的一个调节因子RTE1可与其直接互作，在转录后水平对ETR1进行调节，更多乙烯受体的调节因子以及其在不同层面对乙烯受体的调节机制尚未得到挖掘。申请者所在的研究团队通过EMS化学诱变筛选和图位克隆，得到了乙烯受体ETR1的一个可能的调节因子ABH1。已知ABH1参与RNA前体剪切与5’端加帽等转录加工过程。据此，本课题拟分析ABH1对乙烯受体ETR1的调控是否具有特异性，以及ABH1是否影响ETR1和乙烯信号转导途径中其他组分的内含子剪切和5’加帽修饰的过程，从而较为系统地了解ABH1在RNA转录加工层面对ETR1的分子调节机制，进而为深入了解乙烯受体的分子调控和乙烯信号转导机制提供新的试验数据和理论基础。

As a gaseous hormone, ethylene regulates plant growth and development, as well as plant response to abiotic and biotic stresses. Ethylene receptor is the key player which is responsible for ethylene perception and initiation of ethylene signal transduction pathway. Therefore, it is crucial to elucidate the regulatory mechanism of ethylene receptor function. Previously, RTE1, a positive regulator of ETR1, was reported to be able to interact with ETR1 physically. Using EMS as a mutagen, we screened the novel regulator of ethylene receptor in etr1-2 population. We found that mutation of ABH1 gene could confer ethylene sensitivity in etr1-2, implying that ABH1 is a candidate involving in regulation of ethylene receptor ETR1. It has been documented that ABH1 is a subunit of cap-binding complex (CBC) and plays important role in RNA precursor processing, such as 5’ caping and exon-intron splicing. In this project, we try to figure out if and how ABH1 regulates function of ethylene receptor. The role of ABH1 in ethylene perception will be test to find out if ABH1 specifically modulates ETR1. The transcriptome between etr1-2 and etr1-2 abh1 will be compared to unravel the difference in RNA precusor splicing, especially for the transcript of ethylene receptor and signaling genes. In addtion, the 5’ caping process will be monitored in both etr1-2 and etr1-2 abh1 by RLM-RACE to find out the possible ABH1-targeted genes which are involving in regulation of ETR1-depedent ethylene perception. This work will provide novel evidences for the regulatory mechanism of ethylene receptor and extend our knowledge of the ethylene perception and signaling, and potentially benefit us from the genetic improvement of the economic plants in the future.