基于乏氧-ROS-Leydig细胞自噬途径的补肾活血法治疗LOH作用机制研究

男性迟发性性腺功能减退症（LOH）以雄激素缺乏样症状为主，睾丸Leydig细胞合成与分泌睾酮是复杂的网络调控系统，各因素间相关作用，相互交织在错综复杂的信号通路中，单一通路的调控必然会引起其他通路的紊乱。因此有效缓解雄激素缺乏样症状的方法，应该是恢复睾丸Leydig细胞功能和内环境稳态。线粒体作为多种应激过程的共同通路，可通过乏氧-ROS与Leydig细胞自噬和凋亡通路相偶联，作为治疗靶点具有独特优越性。中医学认为，肾精亏虚和气滞血瘀是LOH的基本病机，肾精亏虚和气滞血瘀可合理解释LOH的四大症候群，气血失和更是乏氧/氧化应激/自噬、凋亡的高度概括。因此以补肾活血法治疗LOH无疑是良好的治疗思路。本项目针对这些可能问题，研究以乏氧-ROS-Leydig细胞自噬为线索，深入阐释补肾活血法治疗LOH的分子机制，为丰富中医药理论的科学内涵、不断提高疗效而服务。

Male late-onset hypogonadism is mainly characterized by androgen deficiency. Testosterone synthesis and secretion by testicular Leydig cells is a complex network regulation system. The correlations among various factors are intertwined in complex signal pathways. The regulation of a single pathway will inevitably lead to the disorder of other pathways. Therefore, the effective way to relieve androgen deficiency symptoms should be to restore Leydig cell function and homeostasis. As a common pathway in a variety of stress processes, mitochondria can be coupled with Leydig cell autophagy and apoptosis pathway through hypoxia-ROS, which has unique advantages as a therapeutic target. According to traditional Chinese medicine, deficiency of kidney essence and stagnation of qi and blood stasis are the basic pathogenesis of LOH. Deficiency of kidney essence and stagnation of qi and blood stasis can reasonably explain the four major syndromes of LOH. Disharmony between qi and blood is a high summary of hypoxia or oxidative stress or autophagy and apoptosis. Therefore, tonifying the kidney and activating blood circulation is undoubtedly a good way to treat LOH. In view of these possible problems, The study takes hypoxia-ROS-Leydig cell autophagy as a clue to deeply explain the molecular mechanism of tonifying kidney and activating blood circulation in the treatment of LOH, in order to enrich the scientific connotation of traditional Chinese medicine theory and continuously improve the curative effect.