土壤全氟化合物（PFCs）污染问题严峻，对其环境行为了解匮乏成为制约PFCs风险控制的瓶颈。生物炭对PFCs污染土壤的原位修复显示良好前景，在修复过程中发挥重要作用的生物炭胶体由于体积微小可成为PFCs的移动载体从而影响其环境归趋与风险，但这一过程及关键机制尚不明确。本研究以环境问题突出的全氟辛酸（PFOA）和全氟辛烷磺酸（PFOS）为研究对象，首先，应用土柱迁移实验、液相色谱-串联质谱检测技术并结合数值模型化分析，揭示生物炭胶体对PFCs在土壤孔隙中迁移动力学行为的影响并阐明关键控制机制。此外，借助蛋白质组学分析技术从分子水平深入揭示生物炭胶体对土壤PFCs的微生物毒性效应的影响机制。将迁移动力学和分子毒理学结果相结合，可为正确评估和控制生物炭土壤PFCs修复技术环境风险、防止PFCs再迁移、PFCs污染生物标志物的开发以及土壤或地下水PFCs修复策略的制定或完善提供理论依据和技术支撑。

Perfluorinated compounds (PFCs) are synthetic chemicals with excellent thermal stability and oxidation resistance. The extensive application of PFCs in human production and living activities make them entered soil environment sustained through many pathways. The limited understanding of their environmental behavior is the bottleneck restricting soil PFCs remediation. Biochar was recommended for soil PFCs remediation increasingly in recent years, due to its benefits in hazardous chemicals immobilization, carbon sequestration and soil productivity. Notably, biochar could release large amount of fine particles (biochar colloid) it owned originally or produced during the environmental aging process. These biochar colloid had strong adsorption ability, and might change the transport behavior and bioavailability of PFCs in soil, which played a key role in their environmental fate and risks. However, the specific process and the key mechanisms are still unclear. In this research, the most concerned PFCs called perfluorooctanoate (PFOA) and perfluorooctane sulfonate (PFOS) were selected to examine the influence of biochar colloid on the transport behavior and toxic effect of PFCs in relation to solution chemistry. Specifically, we will (1) explore the adsorption behaviors and mechanisms of PFCs on soil and biochar colloid, (2) investigate the transport behavior of PFCs with and without the presence of biochar colloid under statured flow conditions, (3) utilize a one-dimensional form of the convection-dispersion equation with two kinetic retention sites and the XDLVO theory to describe the biochar colloid and PFCs transport in soil porous media, and determine the main mechanisms controlling their migration, (4) examine the difference between the toxic effects of dissolved and colloid-bound PFCs, and reveal the molecular mechanism caused the difference through proteomics approach. This research highlights whether biochar colloid could influence the environmental behavior of soil PFCs and what are the key control mechanisms. The results will reveal how to inhibit the migration and remigration of PFCs and improve the safety of biochar land application thus protect the security of groundwater. It will provide theoretical basis and technical support for the development of PFCs protein biomarkers, and the rational improvement or development of soil or groundwater PFCs remediation technology.