常用中药材黄芩已在我国大规模种植，探究黄芩品质变化机制对科学栽培黄芩具有极大的应用价值。黄酮类成分是黄芩的药效物质基础，也是评价黄芩品质的重要指标，揭示黄酮类成分代谢的变化规律及调节机制是阐述黄芩品质变化机制的关键。内生菌可通过调节植物活性氧(ROS)影响植物次生代谢，是影响中药材品质的一类重要因素。前期实验中我们发现内生菌2B可调节黄芩中四种黄酮成分的代谢积累并促使ROS蓄积，但目前尚不清楚内生菌诱导时黄芩黄酮类成分的代谢变化规律及调节机制。因此本项目利用代谢组学手段探究内生菌诱导时黄芩黄酮类成分的代谢变化规律并探讨ROS迸发在黄酮类成分代谢调节中的信号转导作用，在此基础上进一步揭示内生菌诱发黄芩ROS迸发的分子机制，以系统阐述内生菌调节黄芩黄酮成分代谢变化的信号转导通路。本项目将从新的角度揭示黄芩黄酮类成分的代谢调节机制，有助于全面了解黄芩的品质变化机制，为黄芩的科学栽培提供理论依据。

As common Chinese medicinal material Scutellaria baicalensis has been widely cultured in China, it is of great value to explore the mechanism of quality change of S. baicalensis for scientific cultivation. Flavonoids are the material base for clinical efficacy of S. baicalensis, and they are used as index compounds in quality evaluation. The key points for quality control of S. baicalensis are to identify the factors which can induce the metabolic changes of flavonoids in S. baicalensis and to clarify the related regulatory mechanism. As endophyte can make the secondary metabolism of plants changed by inducing reactive oxygen species (ROS) burst, it is considered as an important factor which can influence the quality of Chinese medicinal materials. Our preliminary studies have also found that the metabolism of ROS and four flavonoid compounds in S. baicalensis would change when S. baicalensis was co-cultured with endophyte 2B. However, it is still unknown how the metabolic changes of flavonoids in S. baicalensis occurred when co-cultured with endophyte. Therefore, in this project，we intend to investigate the metabolic changes of flavonoids in S. baicalensis induced with endophyte 2B by metabolomics approach. Meanwhile, we will explore whether the ROS burst plays as signal transduction in this metabolic process. On this basis, the mechanism of ROS burst in S. baicalensis will be further explored to systematically explain the transduction pathway of the metabolism of flavonoids in S. baicalensis induced with endophyte 2B. This research will reveal the mechanism of metabolic changes of flavonoids in S. baicalensis from a new angle. It can not only help us comprehensively understand how the quality of S. baicalensis changes, but also give a scientific guidance for the cultivation of S. baicalensis.