ARF2-PIF5互作单元在ABS3介导植物衰老途径中的功能研究

植物衰老性状对农业生产具有重要的指导意义。植物在衰老过程中主动调控基因表达，调整新陈代谢，重新分配营养物质供给新生组织和贮藏组织，实现后代繁衍和对环境的响应。目前，对植物衰老的转录调控机制的理解还很有限。本项目前期工作中发现ABS3亚家族MATE转运蛋白介导的植物衰老调控途径通过转录因子ARF2和PIF5实现衰老相关的转录重编程，并且ARF2和PIF5之间直接互作。在此基础上，本项目拟通过ARF2和PIF5在衰老调控过程中共同作用的靶基因鉴定，ARF2-PIF5互作在衰老调控中的生物学功能研究，以及ABS3介导衰老途径中ARF2-PIF5功能单元的激活机制解析等三方面工作，揭示ARF2-PIF5互作单元在ABS3介导植物衰老途径中的功能，揭示ARF2和PIF5参与衰老调控的新机制，进一步阐明ABS3介导的植物衰老调控途径的分子机理，为人工调控植物衰老，改良作物的衰老性状提供理论支撑。

Senescence trait is of great importance in crop production. During senescence, plants actively regulate global gene expression, adjust metabolism, and redistribute nutrients to meristems and storage tissues to ensure reproduction success and to adapt to the changing environment. However, current understanding to transcriptional regulatory mechanisms of plant senescence is still very limited. In our previous study, we have discovered that the ABS3 subfamily MATE transporter-mediated senescence pathway involves transcriptional reprogramming through the transcription factors ARF2 and PIF5. Moreover, we demonstrated that ARF2 and PIF5 directly interact. Based on these findings, we propose to explore the function of ARF2-PIF5 interaction module in ABS3-mediated plant senescence pathway on three fronts. First, we will identify the common target genes of ARF2 and PIF5 in senescence regulation. Second, we will uncover the role of ARF2-PIF5 interaction in senescence regulation. Finally, we will analyze the activation mechanism of ARF2-PIF5 interaction module in ABS3-mediated senescence regulation pathway. This work will provide new insights into the role of ARF2 and PIF5 in transcriptional regulation, broaden our understanding to the molecular mechanism of the ABS3-mediated plant senescence pathway, and provide new venues for crop improvement through the artificial manipulation of plant senescence.