拟南芥内质网蛋白AMP1调控蛋白稳态平衡和衰老的分子机制

高等植物衰老的启动和进程对种子发育、世代交替和作物产量至关重要。蛋白稳态平衡的丧失是植物衰老的关键特征之一。蛋白稳态平衡的维持也是植物逆境响应和衰老调控的重要环节，但植物中蛋白稳态平衡和衰老的调控机理还不十分清楚。在前期工作中，本项目组建立了一个黑暗碳饥饿诱导衰老的实验体系，利用这一体系发现拟南芥内质网蛋白AMP1负调控碳饥饿胁迫诱导的蛋白降解和植物衰老过程。本项目拟通过遗传互作分析、蛋白降解过程追踪和转录组分析，揭示AMP1与内质网相关的蛋白稳态平衡调控途径的关系；通过内质网和AMP1蛋白动态变化的观察，阐明AMP1调控碳饥饿胁迫下蛋白稳态和衰老的细胞学机理；通过互作蛋白筛选，鉴定碳饥饿胁迫下AMP1参与的蛋白复合体。这三方面的工作将揭示AMP1响应碳饥饿胁迫，调控蛋白稳态平衡和衰老的分子机制，建立一个新的AMP1介导的植物衰老调控途径，为人工调控植物衰老提供新的基因资源。

The initiation and progression of senescence in higher plants is vital for seed development, life cycle and crop production. The loss of protein homeostasis (proteostasis) is one of the hallmarks of aging in plants. Maintenance of proteostasis is also key to the stress responses and the regulation of senescence in plants. However, the regulation of proteostasis and senescence in higher plants is still not fully understood. In our preliminary work, we developed an assay in Arabidopsis seedlings to examine dark/carbon starvation-induced senescence. Utilizing this assay, we established that Arabidopsis ER protein AMP1 functions as a negative regulator of dark/carbon starvation-induced protein degradation and senescence. In this project, we propose to carry out works on three fronts to understand how AMP1 regulates proteostasis and senescence under carbon starvation. We will combine genetic interaction analysis, monitoring protein degradation pathways and RNA-seq analysis to demonstrate the relationship between AMP1 and ER-related proteostasis pathways. We will track the dynamic changes of ER and AMP1 subcellular localization upon carbon starvation to understand the cellular basis of AMP1’s role. We will screen for AMP1 interacting partners under carbon starvation. Our work will uncover the molecular mechanisms of how AMP1 regulates proteostasis and senescence in response to carbon starvation stress, establish the AMP1-mediated senescence regulating pathway and provide new gene resources for artificial manipulation of senescence in crops.