西南地区蔬菜施磷量高、酸性土壤作为该区域主要土壤类型固磷潜力高、磷肥利用效率低是长期制约该区域蔬菜生产绿色发展的关键因素。挖掘蔬菜生物学潜力是实现可持续磷素管理的有效手段，集约化蔬菜生产条件下能否建立既保障高产、又发挥生物学潜力的适宜根层磷素供应及其机制尚不清楚。本项目以西南地区典型辣椒（茄科）和大白菜（十字花科）为研究对象，营养液培养和土壤培养试验相结合，研究供磷水平对根系磷吸收动力学和有机酸分泌的影响，采用qRT-PCR和P-XANES光谱技术揭示供磷水平对磷吸收和有机酸分泌相关的分子调控机制及有机酸对固定态磷素活化的影响；研究菌根真菌侵染及磷吸收相关基因表达对供磷水平的响应；利用西南酸性土壤上不同磷水平的大田定位试验，研究蔬菜产量、根系形态与磷吸收相关基因表达、根际有机酸分泌与磷活化、菌根侵染及相关基因表达对供磷水平的响应，建立协调满足作物产量需求和发挥生物学潜力的根层磷素管理策略。

That excessive phosphorus (P) fertilizer input, high soil P fixation, and low P utilization efficiency are the key factors which restricts the green development of vegetable production in acid soil of southwest China for a long time. Exploring the biological potential of vegetables roots for efficient P acquisition through rhizosphere manipulation is a promising strategy to realize sustainable P management. However，it is still not clear to what extent the biological potential of roots can be explored by soil P management which aims to optimize vegetable production, and the mechanisms that how soil P management regulates rhizosphere process should be further studied. Therefore, pot and field experiments are conducted in acid soil combined with the research methods from agronomy, root morphology, rhizosphere process, and soil process in pepper (Capsicum annuum L.) and Chinese cabbage (Brassica campestris L.). The pot experiments are conducted to study the effect of P supply on uptake kinetics and organic acid secretion by root, and its molecular mechanisms using qRT-PCR; to study the effects of organic acid secretion on activation of fixed P in acid soils with P K-edge X-ray absorption near edge structure spectroscopy (P-XANES); to study the influence of P supply on mycorrhizal fungi infection and gene expression of P uptake, and reveal the mechanism of mycorrhizal fungal mediated-P uptake in two typical vegetables of southwest China. The field experiment with different P supply levels in acid soils is carried out to study the vegetable yield, root morphology and gene expressions related to P uptake, organic acid secretion and soil P activation, mycorrhizal fungi infection and molecular mechanisms of mycorrhizal fungi mediated-P uptake. The parameters are used to definite soil P management based on root biological potential for efficiency P acquisition and vegetable yield.