乳腺癌是女性最常见肿瘤，转移是导致治疗失败的重要原因，含FERM结构域蛋白是一有40余种成员的大家族，该家族中很多蛋白质被证实与肿瘤发生及转移有关。靶向某些FERM蛋白的小分子药物表现出明显的抗肿瘤转移效果因而具有很好的应用前景。FRMD8是一新的含FERM结构域蛋白，有报道称FRMD8在肺癌癌旁组织中的高表达与肺癌预后不良有关，我们前期的研究发现FRMD8在侵袭性乳腺癌中显著高表达，且该蛋白能与integrin β1相互作用，过表达FRMD8后细胞的运动能力增强。本项目假设FRMD8与乳腺癌的侵袭转移有关，拟通过免疫组化结合临床病理资料分析明确FRMD8在乳腺癌侵袭、转移中的作用，还将在细胞水平上进行功能研究，在分子水平上探讨其促转移是否与integrin激活、EMT及细胞骨架重塑相关，并通过动物实验进行验证。以期揭示该分子在肿瘤转移中的作用及机制，为乳腺癌基因治疗提供新的靶点。

Breast cancer is the most common malignancy in women, metastasis is an important cause of treatment failure. The FERM protein superfamily contains more than 40 members; a number of members were proved to be related to tumorgenesis and metastasis. Small molecule drugs targeting some FERM protein exhibited excellent effect in anti-metastasis therapy and have attractive application prospect. FRMD8 is a novel FERM domain containing protein, one report shown that over-expression of FRMD8 in tissue adjacent to the lung cancer is related to poor prognosis. Our previous research found that expression of FRMD8 in invasive breast cancer is significantly higher than in benign lesions; in addition, this protein can interact with integrin β1 subunit. Up-regulation of FRMD8 enhances cell migration and inhibits cell-ECM adhesion. So we get a hypothesis that FRMD8 promotes breast cancer invasion and metastasis, To prove it, we will verify the effect of FRMD8 to breast cancer invasion by immunohistochemistry detection of pathological samples and analysis of the clinical data. Then we will also investigate the biological functions of FRMD8 , explore whether FRMD5 promotes metastasis through anticipating in regulation of integrin activation, EMT and cytoskeleton remodeling. And animal experiments will be used to further verifying the role of FRMD8 in tumorgenesis and metastasis. Our research will provide new clues for understanding integrin activation, reveal the role of FRMD8 in tumor metastasis, so for as provide new strategies for targeting therapy of breast cancer.