抗逆性是保证作物高产稳产的重要性状，因此小麦抗逆调控基因的挖掘与功能解析具有重要的科学意义和应用价值。在前期工作中，鉴定了一个与小麦抗逆相关的转录因子基因TabZIP15，过表达该基因显著提高了转基因拟南芥对干旱和冻害胁迫的抗性。本项目拟对TabZIP15在小麦中调控非生物抗性的功能机制进行深入研究，包括从转录水平及表达水平分析TabZIP15时空表达特性及在不同逆境胁迫条件下的表达模式；通过对过表转基因小麦基及因编辑材料的表型分析，明确TabZIP15对小麦非生物抗性的调控作用；通过酵母双杂交及ChIP-Seq方法筛选TabZIP15互作蛋白及调控的下游基因；利用小麦微核心种质及其导入系群体，通过关联分析及连锁分析的方法研究TabZIP15基因多样性，挖掘其优异等位基因。通过以上研究以期阐明TabZIP15调控小麦抗逆的功能，丰富小麦抗逆调控的理论，并为小麦新品种培育提供优异基因资源。

The optimum tolerance to abiotic stresses is an important trait for sustainable productivity and high yield potential of crops, therefore, discovering the abiotic stresses tolerance genes and analyzing their function are of theoretical significance and application value. In our previous works, an abiotic stress tolerance related gene TabZIP15 was screened, which can improve the tolerance of transgenic Arabidopsis to abiotic stresses. In this work, we will decipher the effect of TabZIP15 on wheat abiotic stress tolerances through series experiments. The temporal and spatial expression patterns, as well as expression patterns of TabZIP15 respond to abiotic stresses will be characterized at transcription and protein expression level; The regulation of TabZIP15 on wheat abiotic stresses tolerance will be determined based on the analyzing of the transgenic wheat with over-expressing TabZIP15 and the gene editing materials; The interacting protein of TabZIP15 and its direct regulated downstream genes will be screened by Y2H and ChIP-seq methods; The allelic variations of TabZIP15 and their effects on the abiotic stresses tolerance in wheat will be conducted using the wheat mini-core collections and their introgression lines. Through the above research, we hope to uncover the function and molecular mechanisms of TabZIP15 on regulation of abiotic stresses tolerance, and provide new information on the genetic and molecular control of abiotic stresses. And ultimately provide optimum candidate gene for wheat breeding.