叶片在适宜的时期衰老是保证作物高产稳产的重要过程，因此小麦叶片衰老调控基因的挖掘与功能解析具有重要的科学意义和应用价值。在前期工作中，我们通过图位克隆的方法,从小麦EMS早衰突变体m68中克隆到相应候选基因TaLS-2D，超表达该基因可使转基因小麦叶片延迟衰老。本项目拟对TaLS-2D在小麦中调控叶片衰老的功能和分子机制进行深入研究，同时筛选优异等位基因。首先，通过对功能互补和超表达转基因小麦及基因编辑材料的表型分析，明确TaLS-2D对小麦叶片衰老过程的调控作用；其次，通过酵母双杂交方法筛选TaLS-2D的互作蛋白，并利用分子生物学技术对其功能进行验证；最后，通过关联分析及连锁分析的方法研究TaLS-2D基因多样性，挖掘其优异等位基因。通过以上研究以期阐明TaLS-2D在调控小麦叶片衰老过程的功能及其分子机制，丰富小麦叶片衰老调控的理论基础，并为小麦新品种的培育提供优异基因。

Leaf senescence at right time is an important process for high yield production of crops and sustainable agriculture, therefore, to discover genes regulating leaf senescence and to analyze their functions are of theoretical significance and application value in wheat. In our previous works, a candidate gene TaLS-2D regulating the leaf senescence was cloned from an early senescence mutant m68 in wheat EMS mutant library by map-based cloning strategy, and the over-expression of this gene can delay the senescence of transgenic wheat. In this work, we will decipher the effect of TaLS-2D on wheat leaf senescence through series of experiments. Firstly, the role of TaLS-2D on regulating wheat leaf senescence will be determined by analyzing transgenic wheat lines complementary to taLS-2D and the over-expressing TaLS-2D, and knockouting lines generated using gene editing technologies. Secondly, the interacting proteins of TaLS-2D will be screened using Y2H and validated through other molecular biology experiments; Thirdly, the allelic variations of TaLS-2D and their effects on the leaf senescence in wheat will be conducted. Through the research above, we hope to uncover the function and molecular mechanisms of TaLS-2D on regulation of leaf senescence and to provide new information on the genetic and molecular control of leaf senescence, ultimately to utilize the optimum candidate gene identified for wheat breeding to enhance the crop production.