基于AIDS非人灵长类模型研究CD39/CD73-腺苷-腺苷受体轴在病毒库建立与维持中的作用

HIV病毒储藏库是阻止AIDS治愈的关键。目前病毒储藏库标签分子不清楚，制约了靶向病毒储藏库治疗策略的研发。表达免疫检查点分子的细胞既能支持HIV/SIV病毒感染，也能避免宿主免疫反应杀伤，是病毒的重要储藏库。CD39/CD73是重要的免疫检查点分子，中间介质腺苷调节多种免疫反应。目前CD39/CD73-腺苷-腺苷受体轴在病毒库中的作用还不清楚。我们早期的研究发现SIV感染导致CD39表达上调，同时CD39+CD4+T细胞比率与多个疾病进展免疫指标相关。本研究拟采用SIV感染的猕猴样品研究CD39/CD73-腺苷-腺苷受体与疾病进展的关联性，分析CD39/CD73-腺苷-腺苷受体轴与细胞功能耗竭和病毒储藏库的关系，解析CD39/CD73-腺苷-腺苷受体轴在病毒储藏库建立与维持中的作用，探讨干预CD39-CD73腺苷-腺苷受体轴作为AIDS治疗策略的可行性，为AIDS治疗开辟新的治疗途径。

HIV reservior is the key obstacles to the cure of AIDS. At present, the label molecules of virus reservior are not clear, which restricts the development of treatment strategies for eradicating viral reservior. The cells expressing immunocheckpoint molecules can not only support HIV / SIV infection, but also avoid the host immune response, which is an important reservoir of virus. CD39 / CD73 is an important immunocheckpoint molecule. Adenosine mediates regulation a variety of immune responses. At present, the role of CD39 / CD73 adenosine receptor axis in virus reservior is not clear. In our early study, we found that SIV infection resulted in up regulation of CD39 expression, and the frequency of CD39 + CD4 + T cells was related to multiple immune indicators of disease progression. The aim of this study is to study the relationship between CD39 / CD73 adenosine receptor and disease progression, analyze the relationship between CD39 / CD73 adenosine receptor axis and cell function depletion and virus reservior, analyze the role of CD39 / CD73 adenosine receptor axis in the establishment and maintenance of virus reservior, and explore the intervention of CD39/CD73 -adenosine-adenosine receptor axis as a treatment strategy for AIDS.