白纹伊蚊光周期性滞育母代效应传递的分子机制

白纹伊蚊是重要媒介蚊虫，滞育是其实现入侵扩散生物学基础，母代光周期效应是其卵滞育发生决定性因子，但分子机制不明。本课题组前期研究发现其潜在滞育相关分子多存在可变剪切/编辑，常见同一分子在成虫与成虫前期表达模式相反，保幼激素JH参与核心调控，发现滞育激素原pban-dh，提出科学假说：白纹伊蚊光周期性滞育母代效应传递存在复杂多维调控网络，其中光感-神经内分泌信号传导中轴，JH和DH可能协同调控，jhr、dhr、rh7、vri、pdf等分子可能以可变剪切/编辑等多种机制发挥时空特异性核心调控功能。故拟基于三代转录组学及大数据分析和Cas9等功能解析平台，结合GC-MS、ChIP等技术，阐明JH和DH协同调控机制，及其基于可变剪切/编辑的jhr、dhr、rh7、vri、pdf等互作调控网络与机制，探索自亲代阻断滞育发生的新型蚊媒防控途径，亦为昆虫滞育母代效应传递和光周期节律调控通路解析提供借鉴。

Aedes albopictus is a globally distributing vector mosquito that carries varies human pathogens. Diapause is the biologically fundamental to maintain its population and succeed in invasion. The photoperiodic effect is the determining factor for egg diapause of Ae. albopictus, but its molecular mechanism is still unknown. In our previous studies, the existence of alternative splicing/editing of the potential molecules relative with photoperiodic diapause was found by multi omics identification and the expression pattern of the same molecule in adult stage was often contrary to that in pre-adult stages. Moreover, we discovered the existence of prohormone pban-dh and supposed that juvenile hormone III (JHIII) may play an essential role in photoperiodic diapause regulation. All these evidences indicate a more sophisticated regulating network of the maternal effect induced by photoperiod in diapausing of Ae. albopictus. We assume that the photosensitive-neuroendocrine signaling axis may perform its spatiotemporal regulation in multiple mechanisms, such as alternative splicing/editing etc. Therefore, based on advanced technologies including third generation sequencing, the relative big-data analyses and modeling, CRISPR-Cas9, GC-MS，ChIP，and Co-IP，we propose to demonstrate the co-regulation mechanisms of maternal effect of JH and DH during photoperiodic egg diapasue and the photosensitive-neuroendocrine interaction network and mechanisms of multimodal alternative splicing/editing signals, including jhr, dhr, rh7, vri, pdf etc. This study will provide scientific data for exploring a new way to control the mosquito population by blocking the diapause process during the maternal stage in Ae. albopictus, as well benefit for study on maternal effect transmission during photoperiodic diapause and regulation pathway for photoperiodic calendar in other insects or organisms.