从膀胱神经元（BNs）和巨噬细胞（Macs）的互作探讨缩泉丸对OAB膀胱神经稳态的调控机制

膀胱过度活动症（OAB）是常见的下尿路疾病，膀胱神经系统在OAB发病机制中居于核心地位，膀胱神经元（BNs）和巨噬细胞（Macs）之间的互作对调节膀胱功能、维护膀胱神经稳态至关重要。课题组研究发现：缩泉丸治疗OAB的药理作用与膀胱平滑肌功能改善、膀胱神经传导以及神经稳态的恢复有关。基于前期研究基础与科学发现，本项目拟聚焦于膀胱内在的BNs和以Macs为代表的免疫细胞之间的互作调节机制，提出如下科学假说：缩泉丸通过调控Macs和BNs之间的互作，影响膀胱神经元BMPR/Smad1/5/8信号通路以及CSF-1的生成，恢复膀胱神经稳态、调节平滑肌运动，起到治疗OAB的作用。基于这一科学假说，本项目拟采用Single-cell RNA-seq等先进技术，研究缩泉丸对BNs和Macs互作的调控及机制，阐明缩泉丸治疗OAB的药理机制，进一步阐释中医补肾缩尿理论的科学内涵。

Bladder nervous system plays a core role in the pathogenesis of Overactive bladder (OAB), a common lower urinary tract disease. The interaction between Bladder neurons (BNs) and Macrophages (Macs) is crucial for functional regulation and homeostasis maintenance of bladder. Our previous research found that the effect of Suo Quan Wan on OAB was related to the improvement of bladder smooth muscle function, as well as the recovery of bladder nerve conduction and neural homeostasis. Based on these, the project will focus on the interaction between intrinsic BNs and bladder immune cells, represented by Macs, to explore whether Suo Quan Wan would intervene the interaction of these two types of cells in bladder and infect the BMPR/Smad1/5/8 signal pathway and the production of CSF-1 in neurons, by which Suo Quan Wan recover neural homeostasis of bladder, regulate bladder smooth muscle, and then relieve the symptoms of OAB. By using single-cell RNA-seq and the other advanced techniques, the mechanism of Suo Quan Wan on the interaction between Macs and BNs will be investigate to elucidate the pharmacological mechanism of Suo Quan Wan on OAB, as well as provide scientific interpretation for kidney shrinkage urine theory of traditional Chinese medicine.