基于TRPV1通道探讨缩泉丸对膀胱过度活动症的作用机制

以缩泉丸为代表的中医补肾缩尿法在膀胱过度活动症(OAB)的治疗中取得了广泛而可靠的疗效，但缩泉丸干预OAB的具体作用机制尚未十分明确。既往对OAB的研究注重于膀胱逼尿肌及其神经受体功能异常、细胞间连接及信号传递异常等，而对膀胱感觉传入活动的异常有所忽视。瞬时受体电位通道家簇中的TRPV1作为膀胱的压力感受器，维持着膀胱正常排尿功能的机械感觉，同时TRPV1能够调节膀胱的收缩与传入神经的功能，在维护膀胱功能的稳定中具有非常重要的作用。我们前期研究发现，缩泉丸可以调节膀胱逼尿肌舒张功能并且能改善HPA轴功能，因此，我们拟使用缩泉丸对膀胱过度活动症做进一步研究，拟研究调节介导膀胱感觉传入功能的TRPV1的表达及信号转导通路来分析缩泉丸对膀胱过度活动症TRPV1通道的调节作用，初步阐明缩泉丸治疗膀胱过度活动症的具体作用靶点和作用途径，从而明确缩泉丸干预膀胱过度活动症中TRPV1表达的机制。

"Suo Quanwan", as a representative prescription of TCM therapy "nourishing kidney and reducing urination", has significant effect on clinical treatment of Overactive Bladder(OAB), but the mechanism remains unclear. Researches about OAB always focus on functions of destrusor as well as nerve receptors, whether the connection of cells and signaling transmission normal or not,etc. Nevertheless, bladder sensory afferent activities be paid little attention. As a member of transient receptor potential family, TRPV1 is a pressure sensor in the bladder, mediating stretch detection. What's more, TRPV1 can also regulate urinary bladder contractions and function of afferent nerve. It plays a crucial role in maintaining bladder physiological function stability. We found that SQW can adjust bladder detrusor relaxation and improve HPA axis function. Therefore, Our research group plans to further discuss the treatment mechanism of SQW to Overactivity Bladder. TRPV1 which mediate bladder sensory afferent will the breakthrough point. Researching set about from expression of TRPV1 and its signal transduction pathway. We aim to clarify acting targets and pathway of SQW to OAB,and tnen clear the mechanism that SQW can interfere TRPV1 expression of OAB.