DNA甲基化和ncRNAs协同调控番茄果实中乙烯信号途径的机理研究

番茄是研究果实成熟衰老的模式材料，乙烯是番茄果实成熟的启动因子，在番茄果实的成熟衰老过程中起着重要的调控作用。DNA甲基化是一种保守的表观遗传学标记，在植物的生长发育、果实成熟和环境应答的过程中起着重要的调控作用，许多非编码RNAs (ncRNAs) 也参与了番茄果实成熟衰老和乙烯信号途径的调控过程。为了研究DNA甲基化和ncRNAs的协同调控乙烯信号途径的调控功能，我们采用生物信息学和分子生物学相结合的方法，对番茄果实进行全基因组 DNA甲基化和全转录组测序，通过对DNA甲基化和ncRNAs的联合分析，建立起DNA甲基化和ncRNAs的协同调控乙烯信号途径的网络模型，并对DNA甲基化和ncRNAs协同调控乙烯生物合成和信号转导途径的功能进行验证，为进一步阐明番茄果实成熟衰老和乙烯信号途径的机理研究提供理论依据。

Tomato is a model system for studying fruit ripening and senescence. Ethylene is the initiating factor of fruit ripening and plays important roles in regulating the process of fruit ripening and senescence. DNA methylation is a conservative epigenetic marker, which plays an important role in plant growth and development, fruit ripening and environmental responses. Many non-coding RNAs (ncRNAs) also participate in the regulation of tomato fruit ripening and senescence and ethylene signaling pathway. In order to study the regulatory function of DNA methylation and ncRNAs in co-regulation of ethylene signaling pathway, the bioinformatics and molecular biology methods were employed to conduct genome-wide DNA methylation and whole transcriptome sequencing of tomato fruit. Through the joint analysis of DNA methylation and ncRNAs, the network model of DNA methylation and ncRNAs in co-regulation of ethylene signaling pathway would set up. The function of DNA methylation and ncRNAs in synergistic regulation of ethylene biosynthesis and signal transduction pathway would be validated to provide theoretical basis for further elucidating the mechanisms of fruit ripening and senescence and ethylene signaling pathway on tomato fruit.