番茄是研究果实成熟和乙烯的模式材料，其成熟衰老是一个高度协调的遗传调控过程，乙烯是果实成熟衰老的启动因子且乙烯信号途径受很多因子调控。CircRNAs和miRNAs是新发现的转录后调控因子，其功能多种多样，涉及到果实成熟衰老和植物激素调控等许多生理生化过程。为了研究circRNAs和miRNAs协同调控乙烯信号途径的功能，我们采用生物信息学和分子生物学相结合的方法，对番茄果实中circRNAs、转录组、miRNAs进行深度测序，通过对circRNAs和miRNAs靶基因预测和功能分析、circRNAs、mRNA和miRNAs的协同表达关系分析，分离出与乙烯信号途径相关circRNAs，并对circRNAs与miRNAs协同调控乙烯生物合成和信号转导途径的功能进行分析和转基因验证，为最终阐明非编码RNAs参与调控乙烯信号途径的机理奠定理论基础。

Tomato is a model system for study fruit ripening and ethylene, and tomato fruit ripening is a highly harmonious coordination genetic regulation process. Ethylene is the initiation factor of fruit ripening and the ethylene pathway is regulated by many factors. CircRNAs and miRNAs are newly discovered post-transcriptional regulators which play important regulation roles in fruit ripening and plant hormone pathway and all kinds of physiological and biochemical processes. In order to explore the coordinated regulations of circRNAs and miRNAs on ethylene pathway in tomato, circRNAs and transcriptome and miRNAs are chosen for deep sequencing, bioinformatics and molecular biology methods are combined to analysis the targets and the coordinated regulations of circRNAs, mRNA and miRNAs. Identify the circRNAs involved in ethylene pathway and analysis and verify the coordinated regulation functions of circRNAs and miRNAs in ethylene biosynthesis and signal transduction, all of which would lay a theoretical foundation for illuminating the mechanisms of the participation of non-coding RNAs in ethylene signal pathway.