三门湾核电温排水影响区域微生物生态效应研究

核电温排水引起的海水温升对海洋生态系统有显著影响，因而在温排水影响区域需进行全面的环境生态效应评估，以应对温排水的潜在危害。微生物生命周期短，对温度变化敏感，能够显著响应海水温升，可用于温排水生态效应评估。但迄今为止，温排水的微生态效应研究多集中在网采浮游动植物上，对整体及不同粒级微生物群落结构和功能仍缺乏系统的研究。因此，本项目拟从三门湾核电温排水影响区域微生态效应角度出发，综合运用多组学技术，研究微生物整体群落和不同粒级群落结构以及群落功能对温排水的响应特征，量化温排水对微生物群落结构和功能变异的驱动作用，整合温度梯度、季节变化、和核电运营前后的数据，揭示温排水-群落结构-群落功能三者间的关系，进而阐明温排水的微生态效应。通过本研究可为评估温排水对海洋生态系统影响，及国家核电生态安全提供基础资料和理论支撑，为政府部门的管理决策提供依据。

Water temperature increase induced by nuclear power plant thermal discharge can significantly affect marine ecosystems, and thus it is essential to evaluate the ecological effect of thermal discharge to cope its potential risks. Marine microbes are efficient in evaluating the ecological effects of thermal discharge due to their short life cycle and thermo-sensitivity. So far, studies on the micro-ecological effects of thermal discharge mostly focused on large-size plankton, while systematic study on the whole microbial community and their function is still lacking. Based on multi-omics approaches, this project intends to investigate the response of the whole microbial community, microbial sub-community with different particle size, and also microbial community function to thermal discharge, and thus quantifying the effects of thermal discharge on the variation of microbial community structure and function. Meanwhile, by integrating the data from the temperature gradient, seasonal variation, and before and after nuclear power plant operation, this project will deeply elucidate the relationship between thermal discharge and microbial community structure and function, comprehensively revealing the micro-ecological effects of thermal discharge. Overall, this project can provide scientific data for assessing the impact of thermal discharge on marine ecosystems and improving the knowledge of nuclear power ecological safety, and so that it will be helpful to government administration.