海洋细菌在赤潮生消过程中有着重要作用，利用海洋细菌特别是抑藻细菌进行赤潮的防治，是生物法治理赤潮研究的热点。申请人在前期研究中获得一株高效抑藻细菌Hahella sp. KA22，经鉴定其抑藻物质为灵菌红素。灵菌红素是一种典型的细菌抑藻物质，针对其抑藻过程、抑藻范围甚至抑藻机理都有大量报道，但是其抑藻过程的微生态效应还有待深入研究。本项目拟以有毒赤潮藻球形棕囊藻为研究对象，通过构建微宇宙模拟体系，研究灵菌红素在抑藻过程中微生物群落组成、结构和功能的变化规律，揭示灵菌红素抑藻过程中微生物群落的响应特征，进而阐明灵菌红素抑藻的微生态效应；同时筛选抑藻过程中显著变化的微生物类群，作为灵菌红素抑藻生态效应的微生物学指标，为未来全面评估灵菌红素的生态安全性提供坚实的数据支撑，对灵菌红素在赤潮治理中的应用具有重要理论指导和实践意义。

Marine bacteria play important part in the process of harmful algal blooms (HABs), especially bacteria with algicidal activity. Algicidal bacteria have drawn increasing attention for their advantages in coping with HABs threaten. In our previous study, we obtained an algicidal bacterium Hahella sp.KA22, which kills harmful alga Phaeocystis globose by secreting algicide named prodigiosin. Prodigiosin had been proved as a promising algicide in the management of HABs from its algicidal range, process and algicidal mechanism. However, micro-ecological effects in algicidal process of prodigiosin are still not illuminated when it is applied in environment. In this project, we will employ Illumina high throughput sequencing to study the changes of bacterial community composition, structure and function in a P. globose microcosm, exposed to prodigiosin, to reveal the response of microbial community during algicidal process. Then we will combine the microbial community, prodigiosin and environmental variables together to clarify the micro-ecological effects during algicidal process of prodigiosin. Moreover, bacteria taxa with significant changes will be screened as microbial indicator to reflect ecological effect during algicidal process. This study will provide insights into the ecological safety of prodigiosin when it is applied to control HABs in natural waters in the future.