缺氧诱导的circIREB2通过编码多肽IREB2-268aa促进胃癌EMT和转移的机制研究

缺氧是诱导胃癌细胞发生EMT和转移的重要因素。我们前期发现circIREB2在伴淋巴结转移的胃癌组织和缺氧胃癌细胞中高表达，并参与缺氧诱导的胃癌EMT。进一步研究发现circIREB2具有编码多肽的潜能。目前对于circIREB2在缺氧诱导胃癌EMT和转移中的具体作用及机制尚不清楚。基于此，我们提出缺氧诱导circIREB2及其编码多肽的表达上调，经Wnt/β-catenin通路促进胃癌EMT和转移的假设。因此，拟从三个层面进行研究：1、circIREB2在胃癌EMT和转移中的作用；2、circIREB2如何通过编码多肽并参与胃癌EMT和转移的机制；3、circIREB2及其编码多肽作为胃癌治疗靶点和预后指标的潜能。本研究旨在揭示circIREB2在缺氧诱导胃癌EMT和转移中的作用及其机制，为胃癌转移机制提供新的理论依据，亦可为胃癌诊治提供新的潜在靶点，具有重要理论意义和潜在临床应用价值。

Hypoxia is an important factor inducing EMT and metastasis in gastric cancer cells. Our previous research found that circIREB2 which was highly expressed in gastric cancer tissues with lymph node metastasis and hypoxic gastric cancer cells, had been involved in hypoxia-induced gastric cancer EMT. It was also found that circIREB2 had the potency to encode a polypeptide in our further research. The specific role and mechanism of circIREB2 in hypoxia-induced gastric cancer EMT and metastasis remains unknown. We hence propose that hypoxia induces increased expression of circIREB2 and its encoded polypeptide, and promotes EMT and metastasis of gastric cancer via the Wnt/β-catenin pathway. Therefore, we will proceed with our research from the following three layers. 1. The role of circIREB2 in gastric cancer cell EMT and metastasis; 2. How circIREB2 encodes peptides and participates in the mechanism of gastric cancer EMT and metastasis; 3. The potential of circIREB2 and its encoded polypeptides as targets and prognostic indicators of gastric cancer. With significant theoretical and clinical importance, this study which aims to reveal the role and mechanism of circIREB2 in hypoxia-induced gastric cancer EMT and metastasis, will provide new theoretical basis for gastric cancer metastasis, and provide new targets for the prevention and treatment of gastric cancer.