研究胃癌发生发展机制、寻找诊治和预后判断靶点是胃癌研究领域的热点。前期通过TCGA数据库发现GINS4在胃癌组织中表达高于癌旁组织；PCR结果示GINS4在正常胃粘膜、癌前病变、胃癌组织中表达逐渐增高；体外实验发现GINS4促进胃癌细胞生长。为阐明GINS4的调控机制，利用circRNA测序、miRNA芯片和生物信息学分析发现circ_0001790/miR-509-3-5P可能调控GINS4。鉴于此，推测circ_0001790通过海绵样作用吸附miR-509-3-5P，解除其对GINS4的抑制，从而促进胃癌进展。为验证此推测，分别调控三者表达，研究其对胃癌生长的影响；RIP等实验研究GINS4的上、下游调控分子；检测组织和血浆中三者表达与病理和预后的关系。旨在阐明circ_0001790/miR-509-3-5P/GINS4通路在胃癌生长中的作用和机制，发现新的胃癌诊治和预后判断靶点。

In the field of gastric cancer, studying the molecular mechanisms of carcinogenesis and progression of gastric cancer, and searching for diagnosis, treatment and prognosis judgment indicators are becoming the hot topics. In the early stage, it was found that GINS4 expression was obviously higher in gastric cancer than that in the adjacent normal tissue through TCGA data base. Additionally, PCR results indicated that GINS4 expression was gradually increased from normal gastric mucosa, precancerous lesion to gastric cancer tissue. Moreover, in vitro experiments revealed that the growth of gastric cancer cells was promoted by GINS4. To clarify the regulation mechanism of GINS4, we found that circ_0001790/miR-509-3-5P pathway may regulate GINS4 by employing the circRNA sequencing, miRNA microarray and bioinformatics assays. Based on the previous results, we put forward the assumption that circ_0001790 relieves the inhibited function of miR-509-3-5P on GINS4 via sponging miR-509-3-5P, and then promotes carcinogenesis and progression of gastric cancer. In order to verify this speculation, we will separately regulate their expression to study the effect of circ_0001790, miR-509-3-5P and GINS4 on the growth of gastric cancer. RIP and other assays will be applied to study upstream and downstream regulation mechanism of GINS4. The expression of circ_0001790, miR-509-3-5P and GINS4 and their relations with the pathology and prognosis of gastric cancer will be validated separately in tissue and plasma. The purpose of this study is to elucidate the effect and mechanism of circ_0001790/miR-509-3-5P/GINS4 pathway in the growth of gastric cancer, and to provide new molecular biomarkers for diagnosis, treatment and prognosis of gastric cancer.