四神丸干预miRNA-200/ZEB信号调控免疫记忆性T细胞水平预防慢性溃疡性结肠炎复发的作用机制研究

溃疡性结肠炎(UC)难治在于复发已为世人公认，而免疫记忆功能紊乱则是UC复发的重要特征之一，重塑其水平可有效治疗UC，且免疫记忆性T细胞功能受到miRNA-200/ZEB信号调控。我们的前期工作基础表明，四神丸(SSP)可防治UC复发，可能和调节慢性复发性溃疡性结肠炎小鼠免疫记忆性T细胞水平有关，但其调控的作用机制不明。遂提出SSP能通过miRNA-200/ZEB信号调控免疫记忆性T细胞水平预防UC复发的科学假设。于是我们采取miRNA200c基因敲入型小鼠复制慢性复发性UC小鼠，在验证SSP防治慢性UC复发同时，用流式细胞术检测免疫记忆性T细胞分类、亚群和功能等变化，并采用RT-qPCR检测miRNA-200/ZEB通路上核心蛋白表达，进而从miRNA-200/ZEB信号调节免疫记忆T细胞分化角度，明确SSP防治UC复发的可能机制，为中医药防治慢性疾病复发机制提供科学依据。

It is known that Ulcerative colitis (UC) is a refractory and relapse disease.It is an important character of UC which is including that the disbalance of memory T cell, which was controlled by miRNA-200/ZEB signaling pathway. It hints that remodeling memory T cells is an effective pathway to treat UC via miRNA-200/ZEB signaling pathway. In our previous study, Sishen Pill (SSP) prevented the relapse of UC, which was realized by regulating the memory T cell level in the DSS-induced colitis mice. So our scientific hypothesis is whether SSP prevents the relapse of UC by regulating memory T cell on miRNA-200/ZEB signaling pathway. In the present study, we will induce the relapsed colitis on H11-cag-miRNA200c transgenic mice by DSS, while we will verify the effectiveness of SSP prevented UC by pathological observation, and analyze the levels of memory T cell’ subgroup, function, and activation of miRNA-200/ZEB signaling pathway analyzed by RT-qPCR, flow cytometry, western blot and so on. All results will illuminate the mechanism of SSP prevented the relapse of UC by memory T cell on miRNA-200/ZEB signaling pathway. It will be an experimental and scientific evidence which the Traditional Chinese Medicine treated and prevented chronic disease.