橡胶树白粉菌效应蛋白OHEP10与寄主Mlo基因互作机理的研究

白粉菌通过在植物细胞内形成吸器，产生大量的效应蛋白，从而实现在寄主细胞内的侵染。前期文献报道，通过对橡胶树白粉菌进行基因组和转录组测序分析，预测出133个潜在的效应蛋白，然而，这些蛋白的功能是什么，目前还是未知的。本项目前期利用蛋白质组学分析，找到了12个在吸器内高表达的潜在效应蛋白，并且发现效应蛋白OHEP10(Oidium heveae Effector Protein 10)可以促进橡胶树白粉菌的毒性功能。另外，通过酵母双杂交筛选，发现OHEP10与HbMlo12(Hevea brasiliensis Mildew Resistance Locus O 12)可以发生相互作用。本项目将进一步利用生物化学和细胞生物学方法，深入研究OHEP10与Mlo基因的互作机制，探讨OHEP10在植物体内的毒性作用机理，为将来解决白粉菌引起的橡胶树病害提供重要理论基础和潜在的应用价值。

Powdery mildew fungi are obligate biotrophs that secretes a plethora of effectors through haustoria for their nutrition and survival on living host plant cells. Previous study has shown that 133 candidate effectors of Oidium heveae have been predicted by genome and transcriptome sequencing. However, the biological function of these effectors are still unknown. In this project, we found 12 candidate effector proteins of Oidium heveae by proteomes analysis, which are very conservative and display high expression in haustoria. Furthermore, we found that effector OHEP 10 (Oidium heveae Effector Protein 10) can interact with HbMlo 12 (Hevea brasiliensis Mildew Resistance Locus O 12) and enhance the virulence of Oidium heveae in Arabidopsis. Thus, in the project, we will further determine the interaction between OHEP 10 and Mlo by using the Immuno-precipitation and Pull down methods. And, the virulence mechanisms employed by OHEP 10 and Mlo will be explored through genetics, cytology and biochemistry methods, which lay the fundation for the future prevention of powdery mildew in Hevea brasiliensis.