与野油菜黄单胞菌效应蛋白AvrXccC相对应的抗病基因分离及鉴定

AvrXccC是野油菜黄单胞菌Xcc 8004的一个III型分泌效应蛋白。AvrXccC是一个双功能效应因子，在寄主甘蓝上发挥毒性功能促进细菌侵染，但是在芥菜和拟南芥上可以被识别，激活抗病反应。本项目在实验室前期工作的基础上，利用免疫沉淀-质谱的方法分离与AvrXccC发生相互作用的寄主蛋白。从遗传的角度证实AvrXccC是通过其互作蛋白来激活植物抗病反应的。此外，通过体内的免疫共沉淀试验和体外的PULL-DOWN试验以及质谱分析，进一步探索AvrXccC是如何被寄主识别的分子机理。本项目旨在寻找野油菜黄单胞菌效应蛋白的抗病基因，为进一步防治该病菌奠定基础。

AvrXccC is a type III secretion system（TTSS） effector protein of Xcc 8004. This effector is required for full bacterial virulence in the susceptible host cabbage and avirulence in the mustard and Arabidopsis. AvrXccC can be recognized by Resistance proein(s) because AvrXccC-triggered disease resistance and PR1 gene expression in Arabidopsis.So,this project is to identify and characterize the R protein of AvrXccC. The Immunoprecipitation-Tandem mass spectrometry method was used to isolate the AvrXccC interaction proteins(CBPs) in Arabidopsis. And then, whether the AvrXccC-triggered disease resistance and PR1 gene expression are CBPs dependent will be tested, and the molecular mechanisms of AvrXccC is how to be recognized by CBPs will be explored. In conclusion, this project is to identify Xcc R proteins and lay the fundation for the future prevention of this pathogen.