刺参生殖与夏眠生理状态转变的神经内分泌调控特征及机理研究

刺参是我国重要海洋经济物种，产业规模不断扩大，其基础生物学研究受到关注。刺参于初夏完成生殖活动，继而进入“夏眠”，严重影响养殖生产效率。本项目围绕刺参生殖与夏眠生理状态转变这一特殊阶段，在前期刺参G蛋白偶联受体基因预测及多组织转录表达差异分析、相关神经激素信号系统预测与鉴定、刺参成体组织细胞培养方法优化等工作基础上，从神经内分泌研究角度，运用代谢组学、多肽组学及转录组学等高通量测试分析手段，结合分子及细胞生物学和生理功能研究方法，查明刺参生殖与夏眠生理状态转变过程中的神经内分泌调控特征，阐明神经激素与受体的作用活性及介导的细胞信号转导特征，解明关键神经内分泌信号系统的调控关系及其生理功能，综合构建神经内分泌调控网络模型，探明刺参生殖与夏眠状态转变的神经内分泌调控机制。本项目的实施，将从本质上揭示刺参生殖与夏眠转变的内在调控机理，丰富刺参基础生物学研究理论，获得动物生殖与休眠调控研究新认识。

Sea cucumber Apostichopus japonicus is an important marine economic species in China with expanding industrial scale, and a great number of researches are focusing on its basic biological processes. A. japonicus ceases reproductive activities in early summer, and then enters into the "aestivation" status, which seriously affected the production efficiency of aquaculture. Based on obtained results, from the previous studies, the prediction and transcriptional analysis of A. japonicus G protein coupled receptors, the prediction and identification of neurohormonal systems, and the optimization of primary cell culture and tissue culture techniques, this project focuses on the special stage between reproduction and aestivation of A. japonicus from the perspective of neuroendocrine research. Using high-throughput detection and analysis, such as metabolomics, peptidomics and transcriptomics, combined with molecular, cellular and physiological researches, we aim to clarify neuroendocrine regulatory characteristics during the transiting stage, evaluate the interaction between neurohormones and receptors, clarify the characteristics of cell signal transduction, understand the regulatory relationship and physiological functions of key neuroendocrine signal systems, and comprehensively construct the neuroendocrine regulatory network and explore the regulatory mechanism of this model. The implementation of this project will essentially reveal the internal regulatory mechanism of the physiological transition stage in sea cucumber and gain a new understanding of animal reproduction and dormancy control.