5-HTR介导的信号转导对刺参夏眠期间摄食的调控及其机理研究

摄食停止是动物夏眠期间最明显的生理现象之一，深入研究夏眠期间摄食调控机理是揭示夏眠机制的重要内容。本项目以重要海洋经济水产物种刺参为研究对象，在前期研究获得了刺参G蛋白偶联受体（GPCR）基因全长的基础上，采用细胞信号转导研究技术，深入研究刺参5-羟色胺受体（5-HTR）的信号转导和构效机制；利用RNA干扰技术，揭示刺参5-HTR介导的信号转导在刺参摄食调控中的生理功能；通过室内诱导刺参夏眠实验，阐明刺参夏眠期间各组织中5-羟色胺（5-HT）含量及5-HTR基因表达调控过程；综合以上结果，探明5-HTR介导的信号转导对刺参夏眠期间摄食的调控及其机理。本项目的实施，将有助于从本质上认识和揭示5-HTR在刺参夏眠期间摄食调控中的作用机理，获得动物夏眠调控基础理论的新成果；同时将推进海洋生物GPCR研究进展，为海洋生物生理调控研究提供新的思路。

Change of feeding behavior is one of the most obvious physiological regulations in aestivating animal. The study of regulatory mechanism of food intake during aestivation is an important part of the research on molecular mechanism in aestivation. This project is mainly about the study of sea cucumber Apostichopus japonicus on the feeding regulation during aestivation. Based on the G protein-coupled receptor (GPCR) genes obtained in preliminary studies, the in-depth study of signal transduction mechanisms and structure-activity of A. japonicus 5-HT receptor (5-HTR) will be carried out using cellular signal transduction research techniques; the physiological regulatory function of A. japonicus 5-HTR in food intake will be verified and revealed by RNA interference technology; the role of 5-HT and 5-HTR in food intake regulation during A. japonicus aestivation will be recognized through the aestivation inducing experiments in laboratory. With the molecular biological, cell biological, physiological and biochemical research, a comprehensive investigation of the 5-HT and 5-HTR action in ingestion behavior of sea cucumbers will be performed. It will help us to make good understand about the role of 5-HT in food intake regulation during aestivation in A. japonicus and progress in theoretical research achievements of aestivation, it also promotes the GPCR research in marine biology and provides new ideas for the discovery of the physiological regulation mechanism in marine organisms.