微小尺度生物样品淬冷玻璃化行为与逆Leidenfrost效应的协同作用机制

逆Leidenfrost效应普遍存在于微小尺度生物样品淬冷玻璃化过程中，对生物样品冷冻保护质量造成了不利影响，亟待系统探究其作用机制及防止方法。本项目提出微小尺度生物样品淬冷玻璃化行为与逆Leidenfrost效应是具有协同作用的复杂传热传质问题的研究思想，探究逆Leidenfrost效应对微小尺度生物样品玻璃化形成能力的影响。项目拟探索三个方面的基础性关键问题：一是建立微小尺度生物样品淬冷玻璃化及逆Leidenfrost效应关键参数的定量评估方法；二是准确刻画微小尺度生物样品淬冷玻璃化行为与逆Leidenfrost效应之间的协同作用机制；三是探索防止或削弱逆Leidenfrost效应不利影响的有效解决方案。该项目聚焦于微小尺度生物样品玻璃化保存技术瓶颈的关键科学问题探究，对于我国重要生物资源的高通量、高质量、长期保存以及相关基础科学研究和产业需求都具有非常重要的意义。

Inverse Leidenfrost effect generally happens in the process of vitrifying biosamples by quenching with liquid nitrogen, which has an adverse effect on the qualities of cryopreserved biosamples. The current proposal presents a research idea that the synergism between inverse Leidenfrost effect and vitrification behaviors of microscale biosamples while quenching in liquid nitrogen is a kind of complicated heat and mass transfer problem in nature. And it is necessary to assess the effect of inverse Leidenfrost on the vitrification trend and find out an approach to avoid this adverse effect for biosamples long-term storage in low temperature. In current proposal, three critical issues will be mainly concerned such as how to develop the quantitative assessment method for both vitrification degree inside cryopreserved biosamples and key parameters of inverse Leidenfrost phenomenon, how to accurately characterize the synergism mechanism between inverse Leidenfrost effect and vitrification behaviors of biosamples by quenching, and how to eliminate the adverse effect of inverse Leidenfrost effect experimentally for the purpose of cryopreservation application. Importantly, the current proposal aims to figure out the key scientific issues related to vitrifying microscale biosamples by quenching. This should be very helpful for developing high-throughput, high-quality, and long-term storage protocols of important biological resources in China. Also, it can meet the demands in some basic scientific field and pharmaceutical industry.