滋阴清热复方降低ROS/MT-CO1异常与中西医协同治疗SLE互补新机制

研究中西医协同治疗SLE互补机制意义重大。我们证实滋阴清热复方降ROS、GC升ROS；线粒体MT-CO1对ROS损害易感并在狼疮鼠异常下降。ROS为重要促炎分子，ROS与MT-CO1恶性循环可促进炎症。我们推测GC线粒体途径蓄积性加重ROS/MT-CO1恶性循环的促炎作用达阈值后，突破GC细胞核途径抗炎效应的屏蔽，致狼疮活动不定期复发，是GC副作用机理之一；中西药对ROS相反作用是中西医互补机制之一。课题为研究中西医协同治疗SLE互补新机制，首次将ROS/MT-CO1引进SLE领域，线粒体、细胞水平分别干预ROS，从细胞器到整体多层级研究ROS/MT-CO1在SLE中作用，多时段重点研究GC线粒体途径蓄积性加重ROS/MT-CO1异常与狼疮活动复发的关系；中药和GC多组合干预，明确中西医相反调控ROS/MT-CO1对狼疮活动影响；锁定中西医协同治疗SLE互补新机制及GC促炎副作用机理研究。

It is of great significance to study the complementary mechanism of Traditional Chinese Medicine (TCM) and Western Medicine synergistic treatment for SLE. ROS is an important pro-inflammatory molecule. We confirmed that Nourishing Yin Clearing Heat formula can reduce ROS while glucocorticoid (GC) can increase ROS. Mitochondrial cytochrome c oxidase subunit 1 (MT-CO1) was susceptible to ROS damage and decreased abnormally in lupus mice, suggesting a vicious cycle can exist between MT-CO1 and ROS which may promote inflammation. We speculate that GC mitochondrial pathway break through the anti-inflammatory shield of GC nuclear pathway, which can cumulatively aggravate the ROS / MT-CO1 vicious cycle, leading to the irregular recurrence of lupus activity, which is one of the side effects of GC. The reverse regulatory effect of Chinese and Western Medicine on ROS is one of the complementary mechanism of TCM and Western medicine. In order to study the new complementary mechanism of combined Chinese and western medicine treatment of SLE, we first time introduces mitochondrial ROS/MT-CO1 into the field of SLE. We will intervene ROS at the mitochondrial and cellular levels, and the role of ROS/MT-CO1 in SLE was studied from organelle to the whole multilayer level. The periodic observation was used to investigate the relationship between GC accumulation promoting ROS/MT-CO1 abnormalities and lupus activity recurrence. The multi-combination intervention of TCM and GC was used to clarify the effect of ROS/MT-CO1 on lupus activity by the opposite regulation of TCM and western medicine. We Targeted study on the new complementary mechanism of combined Chinese and western medicine treatment of SLE and the mechanism of side effects of GC in promoting inflammation.