我们研究发现：中医滋阴清热药可以降低狼疮鼠抗核抗体和骨髓幼红细胞巨噬细胞连接蛋白（EMP）的增高。骨髓EMP关键性连接幼红细胞和巨噬细胞，决定幼红细胞脱核成熟。脱核后无细胞其他成分的幼红细胞孤核被有抗原呈递功能的巨噬细胞吞噬，孤核满足作为核抗原诱发抗核抗体的理论条件。红斑狼疮核抗原未能定位，一直束缚抗核抗体机理研究。本课题根据前期滋阴清热药对抗核抗体和EMP的作用，提出幼红细胞孤核是核抗原，EMP增高涉抗核抗体产生机理的假说，依滋阴清热法从肾论治狼疮，肾生骨髓之理，将骨髓EMP引入红斑狼疮研究领域，以脂多糖诱发狼疮鼠多重狼疮活动，滋阴清热中药干预，研究滋阴清热法调控骨髓EMP对狼疮鼠抗核抗体和孤核免疫效应的作用，基因沉默EMP等基因，明确中药的作用机理，研究抗核抗体病机中的EMP作用和孤核的核抗原定位。课题目标是突破SLE抗核抗体产生机理不清的困局，推进中医滋阴清热法治疗SLE机理研究。

Our research found that nourishing yin and clearing heat herbs can decrease the lupus mice abnormal increase in anti nuclear antibody and bone marrow erythroblast macrophage protein (EMP). Bone marrow EMP connecte crucially erythroblast and macrophage and determine erythroblast enucleation and maturation. the erythroblast solitary nucleus which have no other components of the cell were phagocytosis by macrophage which has the antigen presenting function, The erythroblast solitary nucleus have an theoretic conditions to be unknown nuclear antigen to anti nuclear antibody. The nuclear antigen of lupus erythematosus has not been located which have always been tied up the study on mechanism of anti nuclear antibodies. According to the nourishing yin and clearing heat traditional Chinese medicine effect on antinuclear antibody and EMP in our research we present hypothesis that solitary nuclear could be the solution in nuclear antigen puzzle, higher EMP involve unknown pathogenesis of lupus anti nuclear antibody. To study effect of Ziyin Qingre method on antinuclear antibody, according to the nourishing yin and clearing heat treatment on lupus from the kidney, kidney affect the bone marrow in TCM theory, we introduce bone marrow EMP into lupus erythematosus research field, with lipopolysaccharide induced murine lupus multiple lupus activity, using nourishing yin and clearing heat medicine intervention. To observe Ziyin Qingre method regulation on EMP pathway effect on nuclear antibody mechanism and the influence on immunological changes of erythroblast solitary nucleus , with in vivo gene silencing EMP gene to explore the effects of Ziyin Qingre method regulation mechanism. study the role of EMP in the pathogenesis of anti nuclear antibodies and the localization of nuclear antigen of erythroblast solitary nucleus. The subject goal is: To break through the dilemma of the mechanism of SLE anti nuclear antibody. and promote the study of TCM lupus erythematosus