hUC-MSCs重编程为汗腺样细胞与多孔羊ADM复合培养制备功能性生物敷料的实验研究

大面积深度烧伤创面修复后，汗腺等附属器不能再生，皮肤丧失排汗功能，严重影响患者的生活质量。本研究通过皮肤组织工程技术联合汗腺样细胞，制备功能性生物敷料，移植覆盖创面，实现严重皮肤损伤的功能修复。脐带间充质干细胞（hUC-MSCs）具有较强的增殖分化潜能，是理想的种子细胞。外胚叶发育不全蛋白基因（EDA）对汗腺的发育形成至关重要，本研究通过慢病毒转染，在hUC-MSCs中过表达EDA，重编程为汗腺样细胞，获得大量种子细胞。羊脱细胞真皮支架（ADM）作为组织再生的天然真皮基质的独特三维结构益于细胞增殖再生，将羊皮制成多孔三维结构的羊ADM，将汗腺样细胞与羊ADM复合培养，研究其与羊ADM复合最佳细胞数量，检测汗腺样细胞在ADM内的生长情况，将其应用修复裸鼠全层皮肤创面，检测汗腺样细胞在新生组织中的再生情况，探讨hUC-MSCs重编程为汗腺样细胞复合羊ADM敷料在修复皮肤附属器的作用机制。

With severe burn injury repaired, sweat glands can not be regenerated, loss of secretory function, greatly affects patient's quality of life. In this study, skin tissue engineering technique was used to combine sweat gland cell-like cells to make a functional biological dressing, cover the wound, achieved the functional reconstruction of skin.Umbilical cord mesenchymal stem cells (hUC - MSCs) have high potential for proliferation and differentiation, to be as a kind of ideal seed cells. Ectodysplasin (EDA) is very important in sweat gland development, then we construct lentiviruses overexpressed EDA,transfect it into hUC-MSCs, induces the reprogramming of hUC-MSCs into sweat gland-like cells, obtain a large number of seed cells. Acellular dermal matrix( ADM) of sheep is a dermal scaffold that retains the three-dimensional structure of normal collagen,which is benefit for cell proliferation. The sheepskin is made into a porous three dimensional sheep ADM, the sweat gland-like cells were combined with the sheep ADM, to test the appropriate number of cells and the growth state of sweat gland-like cells on the ADM, applying it to the full-thickness skin wound of nude mice, detecting sweat gland in the new tissue regeneration，to investigate the mechanism of hUC-MSCs reprogramming into sweat adenoid cells combined sheep ADM dressing in promoting skin function repaired.