严重大面积烧伤救治过程中，临床上多选用生物敷料对创面进行修复。本课题组多年来致力于脱细胞羊皮的开发与应用，前期实验研究中取的了较好的效果。细胞疗法在临床及科研工作中已成为治疗多种疾病的新策略。ADSCs具备自我更新能力与多向分化潜能，同时具有自体取材容易、来源广泛的优势。此外有研究证明，GH和Tβ4具有明显促进创面愈合的作用。本课题拟在体外分离培养脂肪干细胞，一方面将ADSCs诱导成为ECs；另一方面构建hGH和Tβ4的真核表达载体，转染ADSCs，建立转染稳定的ADSCs细胞系。本课题将ECs以及转染的ADSCs细胞系种植于微孔羊皮支架中，构建一种能够分泌促进创面愈合生长因子的复合生物敷料。选用全层损伤模型，将复合羊皮敷料移植于裸鼠皮肤全层损伤处，观察其对皮肤全层损伤创面修复的情况，以及植入的干细胞在体内创伤环境下，细胞存活、生长和分化情况。

Biological dressing is widely used in wound healing. Currently, our group is always committed to the development and application of the acelluar dermal matrix, and we have achieved some good results during early experimental study. Cell therapy has become a new strategy for the treatment of many diseases in clinical and research work. Adipose-derived stem cells(ADSCs) have the ability to self-renewal and multi-directional differentiation potential, and it is easy to take. In addition, further studies have shown that growth hormone(GH) and thymosin β4(Tβ4) has obvious promote wound healing. In this study, ADSCs are isolated and cultured in vitro. On the one hand, ADSCs are differentiated to epidermal cells (ECs); on the other hand, construction of eukaryotic expression vector of human GH and Tβ4 and establishment of its stable transfected ADSCs cell lines.And then, the ECs and stable transfected ADSCs cell lines were cultured on the acellular dermal matrix, constructing a complex dressing capable of secreting growth factors to promote wound healing. Choosing full-thickness cutaneous damage model, the composite dressing are transplanted in nude mice that full-thickness cutaneous damage, to observe the repair effects of the composite dressing, as well as ADSCs implanted in vivo wound environment, cell survival, growth and differentiation .