芜菁蜡质合成基因BrrCER3新的剪接变异体

植物蜡质是覆盖在植物表皮复杂有机混合物的总称，其组分差异在植物抵抗干旱、低温、紫外线辐射等作用机制仍然需要进一步揭示。芜菁作为青藏高原地区传统种植的作物，对高海拔地区的环境具有较强的适应性，并具有蜡粉的表型。前期研究发现芜菁BrrCER3基因与蜡质性状相关并在有蜡粉品种存在BrrCER3-X1/X2两种剪切体。因此，本项目拟通过比较BrrCER3基因可变剪切事件在不同栽培区品种的分布规律，分析品种间形成两种剪切体的成因差异的原因，解析不同剪切体对蜡质组分合成的影响，阐明蜡质组分在植物适应高原环境过程中的作用。本项目的结果将揭示芜菁不同地方品种有无蜡粉性状的遗传基础和以及植物对高原极端环境的适应策略。

Plant cuticles are composite structures that cover the aerial epidermis of plants, and the mechanism of component differences in plant resistance to drought, low temperature and ultraviolet radiation still needs to be revealed further. Turnip, as a traditional crop grown in the Qinghai-Tibet Plateau, with the phenotype of waxy has strong adaptability to high altitude environment. Previous studies found that the BrrCER3 gene from turnip were significantly correlate to the characters of waxy, and two splice variant (BrrCER3-X1/X2) were identified in the material of waxy. In the present project, we aim to compare the distribution patterns of two splice variant, analyze the reasons for the differences in the formation of two splice variant and the effect of splice variant on the synthesis of waxy components, and clarify the role of waxy components in the process of plant adaptation to high altitude environment. Our results would provide the genetic mechanisms of characters of waxy among landraces，and the adaptation strategies of plants to the extreme environment on the plateau.