青藏高原针茅属植物蜡质合成基因WSD1功能域的位点变异及其对耐旱性分化的影响研究

植物对极端环境耐受力的差异塑造了植物的分布格局，但其机制仍然需要进一步揭示。青藏高原生长季节的降雨由东南向西北逐渐降低，为研究植物在长期适应不同的干旱环境过程中正选择基因的筛选及其功能研究提供了理想的材料。青藏高原的丝颖针茅、紫花针茅和沙生针茅由东向西沿水分降低的梯度分布呈现替代分布，前期研究发现植物叶片表面蜡质合成相关的WSD1基因在紫花针茅和沙生针茅受到正选择并响应干旱处理，表明这一基因可能与植物的抗旱能力密切相关。因此本项目拟对三种针茅属植物的WSD1基因进行全长克隆、基因正选择位点突变、蛋白活性检测以及转基因功能研究，分析受正选择WSD1基因与植物蜡质合成能力，阐明WSD1基因在不同针茅属植物响应或适应不同干旱环境过程中的作用。本项目的研究结果既能揭示非模式植物对极端环境的耐受机制以及植物分布格局的形成机制，又能为青藏高原植物的基因资源研究提供依据。

Differences in tolerance of plants to extreme environment shape the distribution pattern of plants, but its mechanism still needs to be revealed further. The precipitation gradually decreased from the southeast to the northwest in the Tibetan Plateau, which provides an ideal material for the study of genes in the process of long-term adaptation of plants to different drought conditions. Stipa capillacea, Stipa purpurea and Stipa glareosa distributed along a moisture gradient from the southeast to the northwest in the Tibetan Plateau. Previous studies found that plant leaf surface wax synthesis related WSD1 gene of S. purpurea and S. glareosa have been shown to be driven by positive selection and induced by drought treatment, suggesting that this gene may be closely related to the drought resistance of the plant. In the present project, we aim to analyze the relationship between the WSD1 gene and plant wax synthesis ability, and to clarify the mechanism that WSD1 improve the resistance of Stipa species to drought stresses by cloning full length of WSD1 gene, gene positive selection site mutation, protein activity detection and gene function research, and it can provide the basis for the study of the genetic resources of plants in Tibetan Plateau.