湖泊富营养化对浮游病毒与宿主相互关系的影响及机理研究

湖泊富营养化已成为全球面临的重大挑战。研究湖泊浮游病毒与宿主互作模式有利于深入理解微食物环和元素生物地球化学循环过程。遗憾的是，目前有关营养水平影响病毒-宿主互作的研究局限于海洋，且未形成统一观点，对湖泊中这一问题的认识十分有限。本研究瞄准这一短板，将营养水平与病毒-宿主互作紧密结合，对不同营养水平湖泊开展病毒-宿主互作模式的调查和机制研究。选取云南不同营养水平的高原湖泊，现场调查病毒感染率，爆发量，溶原率等，了解其与环境因子的相关性；通过室内条件下不同营养水平湖泊微生物置换和贫营养水样加富实验揭示富营养化与病毒-细菌互作模式的因果关系；以分离的噬菌体-宿主体系研究磷富集如何影响噬菌体-宿主互作模式，揭示磷浓度升高对病毒-宿主的作用机制。我们的研究将明确不同营养水平高原湖泊中病毒-宿主互作模式，提升对营养水平如何影响湖泊病毒生态功能的认识。

Lake eutrophication has become a major challenge facing the world. Studying the interaction model of lake planktonic virus and its host is conducive to in-depth understanding of the microbial loop and element biogeochemical cycling process. However, current research on the effects of nutritional levels on virus-host interactions were limited in the ocean, and a unified view has not been formed, and few touched on lakes. The present study will focus on this shortcoming, and closely integrates the nutrition level with virus-host interaction, and investigates the interaction model and mechanism of virus-host interaction patterns in lakes with different nutrition levels. we will investigate 1)the virus infection rate, burst size, lysogenic rate of Yunnan plateau lakes with different nutrition levels, and their correlation with environmental factors;2) the cause-and-effect relationship between eutrophication and virus-bacteria interaction by the experiments of microbial replacement lakes with different nutrient levels and enrichment of nutrient-deficient water samples;3) the influence and mechanism of the enrichment of phosphorus on the phage-host interaction pattern. Our research will clarify the virus-host interaction pattern in plateau lakes with different nutritional levels, and enhance the understanding of how nutritional levels affect the ecological function of lake viruses.